[牛生长激素在大肠杆菌DH1细胞中的生物合成及其中试规模的纯化]

[Biosynthesis of bovine growth hormone in Escherichia coli DH1 cells and its purification on a pilot scale].

作者信息

Nikolaeva V M, Bykovskaia S V, Kozhin V A, Maĭorov S M, Usankina T G, Rukhlova L G, Luzina I E, Ezhov V A

出版信息

Prikl Biokhim Mikrobiol. 1993 Jan-Feb;29(1):150-4.

Abstract

The biosynthesis of the bovine growth hormone (BGH) in E. coli DH1 cells containing the plasmid pbGH(1-119)ptrp was studied. It was found that BGH accumulated in the cytoplasm of bacterial cells as optically dense granules that can be isolated by low-speed centrifugation. A fraction of purified protein granules was used for further purification of BGH. A purified BGH preparation contained two polypeptides with molecular weights of 18,000 and 22,000 D. The state of plasmid was investigated during cultivation and storage of the culture.

摘要

对含有质粒pbGH(1-119)ptrp的大肠杆菌DH1细胞中牛生长激素（BGH）的生物合成进行了研究。发现BGH以光学致密颗粒的形式积累在细菌细胞的细胞质中，可通过低速离心分离。一部分纯化的蛋白质颗粒用于BGH的进一步纯化。纯化的BGH制剂包含两种分子量分别为18,000和22,000 D的多肽。在培养物的培养和储存过程中研究了质粒的状态。