Kurosawa S, Matsuzaki G, Harada M, Ando T, Nomoto K
Department of Immunology, Kyushu University, Fukuoka, Japan.
Eur J Immunol. 1993 May;23(5):1029-33. doi: 10.1002/eji.1830230507.
We investigated NK cell infiltration into tumor developing lesions at early stage of tumor development after intraperitoneal inoculation of 3LL lung carcinoma into syngeneic C57BL/6 mice. Natural killer (NK) cells, which were detected by anti-NK 1.1 monoclonal antibody (mAb), remarkably increased in number in tumor-developing lesions (peritoneal cavity) as early as day 3 after inoculation of 3LL. The tumor-infiltrating NK cells from 3LL-inoculated mice produced a high level of interferon-gamma by co-culture with 3LL and showed enhanced cytotoxic activities against both NK-sensitive (YAC-1) and NK-resistant (3LL and P815) tumors. Furthermore, mice depleted of NK cells by injection of anti-NK 1.1 mAb or anti-asialo GM1 antibody showed shorter survival times after intraperitoneal inoculation of 3LL when compared with control mice. These results suggest that NK cells infiltrate the tumor-developing lesion at an early stage and may participate in the early protection against tumors through production of a high amount of interferon-gamma and enhanced cytotoxicity at tumor-bearing sites.
我们将3LL肺癌腹腔接种到同基因C57BL/6小鼠体内，研究了肿瘤发生早期阶段自然杀伤（NK）细胞向肿瘤发生病变部位的浸润情况。用抗NK 1.1单克隆抗体（mAb）检测到的NK细胞，早在接种3LL后第3天，肿瘤发生病变部位（腹腔）的数量就显著增加。来自接种3LL小鼠的肿瘤浸润NK细胞与3LL共培养时产生高水平的γ干扰素，并对NK敏感（YAC-1）和NK抗性（3LL和P815）肿瘤均表现出增强的细胞毒性活性。此外，与对照小鼠相比，注射抗NK 1.1 mAb或抗去唾液酸GM1抗体使NK细胞耗竭的小鼠在腹腔接种3LL后存活时间更短。这些结果表明，NK细胞在早期浸润肿瘤发生病变部位，并可能通过在荷瘤部位产生大量γ干扰素和增强细胞毒性来参与早期抗肿瘤保护。
