在转染的小鼠神经母细胞瘤细胞中，细胞间接触部位N-CAM的聚集并不需要大的细胞质结构域。

The large cytoplasmic domain is not required for concentration of N-CAM at cell-cell contacts in transfected mouse neuroblastoma cells.

作者信息

Woo M K, Kil S H, Adams L D, Nguyen T N, Murray B A

机构信息

Department of Developmental and Cell Biology, University of California Irvine 92717.

出版信息

Exp Cell Res. 1993 Apr;205(2):331-6. doi: 10.1006/excr.1993.1094.

DOI:10.1006/excr.1993.1094

PMID:8482339

Abstract

We examined the localization of the 140- and 180-kDa transmembrane isoforms of chicken N-CAM following transfection into mouse N2A neuroblastoma cells. Both isoforms were expressed at the cell surface and became partially or completely localized at areas of cell-cell contact after several days of culture or of in vitro differentiation. These results indicate that the presence of the large cytoplasmic domain of the 180-kDa N-CAM isoform is not necessary to bring about the localization of N-CAM to points of cell-cell contact.

摘要

我们将鸡N-CAM的140 kDa和180 kDa跨膜异构体转染到小鼠N2A神经母细胞瘤细胞后，检测了它们的定位情况。培养数天或体外分化后，这两种异构体均在细胞表面表达，并部分或完全定位于细胞-细胞接触区域。这些结果表明，180 kDa N-CAM异构体的大细胞质结构域的存在对于N-CAM定位于细胞-细胞接触点并非必需。