Bavykin S, Srebreva L, Banchev T, Tsanev R, Zlatanova J, Mirzabekov A
Institute of Molecular Biology, Russian Academy of Sciences, Moscow.
Proc Natl Acad Sci U S A. 1993 May 1;90(9):3918-22. doi: 10.1073/pnas.90.9.3918.
An immunochemical method for analyzing protein interactions with BrdUrd-substituted DNA was used to study binding of histones to nascent DNA in nuclei. The results indicate that in Ehrlich ascites tumor (EAT) cells, histone H1 deposits on newly replicated DNA simultaneously with or immediately after core histone deposition so that in chromatin replicated for 3 min, the stoichiometry of the histones is the same as in bulk chromatin. All histones, and especially histone H1, interact with nascent DNA more weakly than with bulk chromatin, although the efficiency of interaction via the globular domains seems to be the same for both types of chromatin.
一种用于分析蛋白质与溴脱氧尿苷(BrdUrd)取代的DNA相互作用的免疫化学方法被用于研究细胞核中组蛋白与新生DNA的结合。结果表明,在艾氏腹水瘤(EAT)细胞中,组蛋白H1在核心组蛋白沉积的同时或之后立即沉积在新复制的DNA上,因此在复制3分钟的染色质中,组蛋白的化学计量与整体染色质中的相同。所有组蛋白,尤其是组蛋白H1,与新生DNA的相互作用比与整体染色质的相互作用更弱,尽管通过球状结构域的相互作用效率对于两种类型的染色质似乎是相同的。