大鼠细胞因子合成抑制因子（IL-10）cDNA的分子克隆及其在脾脏和巨噬细胞中的表达。

Molecular cloning of rat cytokine synthesis inhibitory factor (IL-10) cDNA and expression in spleen and macrophages.

作者信息

Feng L, Tang W W, Chang J C, Wilson C B

机构信息

Department of Immunology, Scripps Research Institute, La Jolla, CA 92037.

出版信息

Biochem Biophys Res Commun. 1993 Apr 30;192(2):452-8. doi: 10.1006/bbrc.1993.1436.

DOI:10.1006/bbrc.1993.1436

PMID:8484757

Abstract

Rat cytokine synthesis inhibitory factor (IL-10) was cloned. Like mouse and human IL-10 (mIL-10, hIL-10), rat IL-10 exhibits strong DNA and amino acid sequence homology to the open reading frame in the Epstein-Barr virus, BCRFI. The supernatant of COS-7 transfectants with rat IL-10 was found to inhibit the production of IFN-gamma by murine Th1 cells. A probe from the rat IL-10 was used in an RNase protection assay to demonstrate that IL-10 was expressed in LPS-stimulated rat spleen and FACS-purified OX-42-reactive peritoneal macrophages. The production of IL-10 by macrophages, as shown in the current study, suggests that IL-10 may have an autocrine function in inflammation.

摘要

大鼠细胞因子合成抑制因子（IL-10）被克隆出来。与小鼠和人IL-10（mIL-10，hIL-10）一样，大鼠IL-10与爱泼斯坦-巴尔病毒BCRFI中的开放阅读框表现出很强的DNA和氨基酸序列同源性。发现用大鼠IL-10转染的COS-7细胞的上清液可抑制小鼠Th1细胞产生IFN-γ。用大鼠IL-10的探针进行核糖核酸酶保护试验，以证明IL-10在脂多糖刺激的大鼠脾脏和经荧光激活细胞分选术纯化的OX-42反应性腹腔巨噬细胞中表达。如本研究所示，巨噬细胞产生IL-10表明IL-10可能在炎症中具有自分泌功能。