亚硫酸盐在体外以及在静脉注射二氯乙酸的雄性大鼠体内，均可抑制由乙醇酸和乙醛酸生成草酸盐的过程。

Sulfite inhibits oxalate production from glycolate and glyoxylate in vitro and from dichloroacetate infused i.v. into male rats.

作者信息

Sharma V, Schwille P O

机构信息

Division of Experimental Surgery, University of Erlangen, Germany.

出版信息

Biochem Med Metab Biol. 1993 Apr;49(2):265-9. doi: 10.1006/bmmb.1993.1028.

DOI:10.1006/bmmb.1993.1028

PMID:8484965

Abstract

The effect of sulfite on oxalate production from glycolate and glyoxylate was investigated in an in vitro assay system using rabbit muscle LDH and rat liver 35-60% ammonium sulfate fraction as enzymes. A > or = 50% inhibition in oxalate production was observed at sulfite to glyoxylate ratio of 0.4. LDH activity (change in A340 nm) was inhibited by > or = 40% at sulfite to glyoxylate ratio of 0.5. Male Sprague-Dawley rats fasted for 24 h and infused with dichloroacetate (150 mg/rat/h, iv, n = 6), without and with sodium sulfite (150 mg/rat/h, n = 6) excreted oxalate in urine, respectively, at the rate of 1.13 +/- 0.29 and 0.61 +/- 0.09 mumol/h/100 g body wt, showing a nearly 50% reduction due to sulfite. The present report demands further experimentation to define the role of sulfite as an intracellular modulator of oxalate production.

摘要

在一个体外分析系统中，使用兔肌肉乳酸脱氢酶（LDH）和大鼠肝脏35 - 60%硫酸铵分级分离物作为酶，研究了亚硫酸盐对由乙醇酸和乙醛酸生成草酸盐的影响。当亚硫酸盐与乙醛酸的比例为0.4时，观察到草酸盐生成受到≥50%的抑制。当亚硫酸盐与乙醛酸的比例为0.5时，LDH活性（A340 nm的变化）受到≥40%的抑制。禁食24小时并分别静脉注射二氯乙酸（150 mg/大鼠/小时，n = 6），以及同时注射亚硫酸钠（150 mg/大鼠/小时，n = 6）的雄性Sprague - Dawley大鼠，尿中草酸盐排泄率分别为1.13±0.29和0.61±0.09 μmol/小时/100克体重，表明由于亚硫酸盐作用草酸盐排泄率降低了近50%。本报告需要进一步实验以确定亚硫酸盐作为草酸盐生成细胞内调节剂的作用。