Safonova I G, Sviridov D D, Rampal' P, Nano Zh L, Pavlov M Iu, Repin V S
Laboratory of Gastroenterology and Nutrition, Faculty of Medicine, Nice University, France.
Biokhimiia. 1993 Feb;58(2):274-84.
Cholesterol absorption in human small intestine organ culture and rat small intestine epithelial cell culture IRD-98 has been studied using [14C] cholesterol, [3H] cholesterol and [14C] sitosterol. It has been found that cholesterol absorption is a dose- and time-dependent process, while sitosterol absorption is not and makes up to about 25% of the total cholesterol absorption. Cholesterol absorption appeared to be a specific process. The endocytosis inhibitor monensin decreased specific cholesterol absorption by 37%. Cholesterol absorption was examined under different conditions influencing cholesterol metabolism in the cell. Loading of IRD-98 cells with non-lipoprotein cholesterol caused a dose-dependent decrease of cholesterol absorption. The inhibitor of acyl coenzyme A:cholesterol acyl transferase (ACAT), compound Sandoz 58-035, had a similar effect on cholesterol absorption. Lovastatin, an inhibitor of 3-hydroxymethyl-3-glutaryl coenzyme A (HMG-CoA) reductase, stimulated cholesterol absorption in a dose-dependent manner. Loading of cells with cholesterol, lovastatin and Sandoz 58-035 had no effect on sitosterol absorption. The possibility has been demonstrated of using human small intestine organ culture and rat small intestine epithelial cell culture IRD-98 as models for studying cholesterol absorption.
已使用[14C]胆固醇、[3H]胆固醇和[14C]谷甾醇对人小肠器官培养物和大鼠小肠上皮细胞培养物IRD - 98中的胆固醇吸收进行了研究。已发现胆固醇吸收是一个剂量和时间依赖性过程，而谷甾醇吸收则不是，且谷甾醇吸收约占总胆固醇吸收的25%。胆固醇吸收似乎是一个特定过程。内吞作用抑制剂莫能菌素使特异性胆固醇吸收降低了37%。在影响细胞内胆固醇代谢的不同条件下对胆固醇吸收进行了检测。用非脂蛋白胆固醇加载IRD - 98细胞导致胆固醇吸收呈剂量依赖性降低。酰基辅酶A：胆固醇酰基转移酶（ACAT）抑制剂化合物山德士58 - 035对胆固醇吸收有类似作用。3 - 羟基 - 3 - 甲基戊二酰辅酶A（HMG - CoA）还原酶抑制剂洛伐他汀以剂量依赖性方式刺激胆固醇吸收。用胆固醇、洛伐他汀和山德士58 - 035加载细胞对谷甾醇吸收没有影响。已证明将人小肠器官培养物和大鼠小肠上皮细胞培养物IRD - 98用作研究胆固醇吸收模型的可能性。
