Kolosova I A, Bernkhard I, Orlov S N, Biuller F R
Biokhimiia. 1993 Mar;58(3):456-60.
The effects of NaF and AlCl3 on basal and stimulated by hyperosmotic shock activities of Na+/H(+)-exchange and Na+, K+, 2Cl(-)-cotransport in rat smooth muscle and red blood cells have been studied. Preincubation of cells with 10 mM NaF leads to inhibition of basal and hyperosmotic shock-activated Na+, K+, 2Cl(-)-cotransport in smooth muscle cells--by 45% and 190%, respectively. Sodium fluoride causes 80% activation of basal Na+, K+, 2Cl(-)-cotransport in red blood cells with no effect on the transport induced by hyperosmotic shock. The effect of NaF on Na+, K+, 2Cl(-)-cotransport in red blood cells does not depend on Al3+. Basal Na+/H(+)-exchange is stimulated by NaF by 780% in smooth muscle cells and is insensitive to the fluoride in red blood cells. Under hyperosmotic conditions NaF activates Na+/H(+)-exchange by 100% in smooth muscle cells and by 130% in red blood cells; in the latter case its effect is potentiated by Al3+. The data obtained suggest that GTP-binding proteins are involved in the activation of Na+/H(+)-exchange under hyperosmotic conditions.
研究了氟化钠（NaF）和氯化铝（AlCl3）对大鼠平滑肌细胞和红细胞中Na+/H(+)交换以及Na+、K+、2Cl(-)协同转运的基础活性和高渗休克刺激活性的影响。用10 mM NaF预孵育细胞会导致平滑肌细胞中基础和高渗休克激活的Na+、K+、2Cl(-)协同转运受到抑制，分别抑制45%和190%。氟化钠使红细胞中基础的Na+、K+、2Cl(-)协同转运激活80%，而对高渗休克诱导的转运无影响。NaF对红细胞中Na+、K+、2Cl(-)协同转运的作用不依赖于Al3+。基础Na+/H(+)交换在平滑肌细胞中被NaF刺激780%，而在红细胞中对氟不敏感。在高渗条件下，NaF使平滑肌细胞中的Na+/H(+)交换激活100%，使红细胞中的Na+/H(+)交换激活130%；在后一种情况下，其作用被Al3+增强。所得数据表明，GTP结合蛋白参与了高渗条件下Na+/H(+)交换的激活。
