Smith M J, Basora T, Kieran J E, Nieto M C
Department of Biological Sciences, California State University, Hayward 94542.
Immunol Cell Biol. 1993 Apr;71 ( Pt 2):145-9. doi: 10.1038/icb.1993.16.
The utilization of the beta 2-microglobulin (B2m) exchange assay allowed for the association of H-2Ld with human B2m. Upon association with H-2Ld, human B2m induces structural alterations in H-2Ld that appear dependent upon xenogeneic B2m amino acid sequence variability. In this regard, xenogeneic B2m exchange is used as a tool to induce structural alterations in class I as a means of analysing the structural-functional relationship of B2m/class I association. Incorporating H-2Ld site-directed mutants into the experimental approach provided strong evidence that B2m makes indirect contact with discrete class I specific amino acid positions located in the helical region of the alpha 1 domain.
β2-微球蛋白（B2m）交换分析的应用使得H-2Ld与人类B2m相关联。与H-2Ld结合后，人类B2m会诱导H-2Ld发生结构改变，这种改变似乎依赖于异种B2m氨基酸序列的变异性。在这方面，异种B2m交换被用作一种工具，通过诱导I类分子结构改变来分析B2m/I类分子结合的结构-功能关系。将H-2Ld定点突变体纳入实验方法提供了有力证据，表明B2m与位于α1结构域螺旋区域的离散I类特异性氨基酸位置进行间接接触。
