类支原体生物（MLOs）并将它们与其他类支原体生物区分开来。

Mycoplasmalike organisms (MLOs) and distinguish them from other MLOs.

作者信息

Nakashima K, Kato S, Iwanami S, Murata N

机构信息

Tropical Agriculture Research Center, Tsukuba, Ibaraki 305, Japan.

出版信息

Appl Environ Microbiol. 1993 Apr;59(4):1206-12. doi: 10.1128/aem.59.4.1206-1212.1993.

DOI:10.1128/aem.59.4.1206-1212.1993

PMID:8489230

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC202262/

Abstract

DNA of 10 lines of rice yellow dwarf (RYD) mycoplasmalike organisms (MLOs) from Japan, the Phillippines, and Thailand hybridized with four probes containing chromosomal and six probes containing extrachromosomal DNA of a Tochigi (Japan) line of RYD MLO. One chromosomal probe (RYD9) and all six extrachromosomal probes hybridized with various other MLOs (sugarcane white leaf, onion yellows, cineraria witches'-broom, Japanese hornwort witches'-broom, water dropwort wiches'-broom, gentian witches'-broom, udo dwarf, tsuwabuki witches'-broom, pelargonium witches's-broom, peach western-X, and pear decline). DNA from the culturable mollicutes Spiroplasma kunkelii, Spiroplasma citri, Mycoplasma hominis, and Mycoplasma orale did not hybridize with RYD MLO probes. The extrachromosomal DNAs hybridizing with the probes showed variations in electrophoretic behavior.

摘要

来自日本、菲律宾和泰国的10株水稻黄矮（RYD）类支原体生物（MLO）的DNA，与含有枥木（日本）株RYD MLO染色体DNA的4个探针以及含有其染色体外DNA的6个探针杂交。一个染色体探针（RYD9）和所有6个染色体外探针与其他各种MLO（甘蔗白叶、洋葱黄化、瓜叶菊扫帚病、日本金鱼藻扫帚病、水芹扫帚病、龙胆扫帚病、土当归矮化、贴梗海棠扫帚病、天竺葵扫帚病、桃西部-X和梨衰退病）杂交。可培养的柔膜菌纲菌属，如玉米螺原体、柑橘螺原体、人型支原体和解脲脲原体的DNA不与RYD MLO探针杂交。与探针杂交的染色体外DNA在电泳行为上表现出差异。