Engelmann K, Drexler D, Draeger J, Böhnke M
Universitäts-Augenklinik Hamburg.
Ophthalmologe. 1993 Apr;90(2):166-70.
Human donor corneas that were unsuitable for transplantation were denuded of their endothelium and then used as substrates for seeding in vitro cultured human or porcine corneal endothelial cells. Only cells that expressed an endothelial-like morphology in vitro formed a monolayer on the denuded matrix whereas fibroblast-like cells showed no redifferentiation. The maximal achieved cell density was 3,500 cells/mm2 if porcine cells were used and 1,800 cells/mm2 for human cells. Adhesion of the cells was best if the subendothelial matrix was carefully prepared mechanically. Moreover, cell seeding was improved by slight centrifugation. Fibroblast growth factor (FGF), which enhanced endothelial cell proliferation in vitro, did not influence proliferation after cell transplantation, but seemed to allow better regeneration of the newly formed monolayer on the corneas.
不适用于移植的人类供体角膜被去除内皮，然后用作接种体外培养的人类或猪角膜内皮细胞的基质。只有在体外表现出内皮样形态的细胞才能在去除内皮的基质上形成单层，而成纤维细胞样细胞则没有再分化。如果使用猪细胞，最大达到的细胞密度为3500个细胞/mm²，如果使用人类细胞，则为1800个细胞/mm²。如果小心地通过机械方法制备内皮下基质，细胞的黏附效果最佳。此外，轻微离心可改善细胞接种。成纤维细胞生长因子（FGF）在体外可增强内皮细胞增殖，但在细胞移植后不影响增殖，但似乎能使角膜上新形成的单层更好地再生。
