Caricasole A, Ward A
Cancer Research Campaign Growth Factors (Zoology) Oxford, Department of Zoology, University of Oxford, UK.
Nucleic Acids Res. 1993 Apr 25;21(8):1873-9. doi: 10.1093/nar/21.8.1873.
The mouse insulin-like growth factor II gene (Igf2) is transcribed from three promoters (P1, P2 and P3), and is expressed in a tissue-specific and developmentally regulated fashion; however, little information is available on the transcription factors controlling Igf2 expression. The AP-1 complex is a transcription factor involved in the regulation of a variety of genes, including those encoding certain growth factors. We show that Igf2 P3 is transactivated by AP-1 in a transient expression assay, and that this effect is mediated through two non-consensus AP-1 binding sites characterised by DNA-protein interaction studies. Mutational analysis indicates these sites are required for AP-1 responsiveness and full promoter activity.
小鼠胰岛素样生长因子II基因（Igf2）由三个启动子（P1、P2和P3）转录，并以组织特异性和发育调控的方式表达；然而，关于控制Igf2表达的转录因子的信息却很少。AP-1复合物是一种参与多种基因调控的转录因子，包括那些编码某些生长因子的基因。我们发现在瞬时表达试验中，AP-1可反式激活Igf2 P3，并且这种效应是通过DNA-蛋白质相互作用研究确定的两个非共有AP-1结合位点介导的。突变分析表明这些位点是AP-1反应性和完整启动子活性所必需的。
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