果蝇 slit 和 Toll 基因的中枢神经系统中线增强子。

CNS midline enhancers of the Drosophila slit and Toll genes.

作者信息

Wharton K A, Crews S T

机构信息

Molecular Biology Institute, University of California, Los Angeles 90024.

出版信息

Mech Dev. 1993 Mar;40(3):141-54. doi: 10.1016/0925-4773(93)90072-6.

DOI:10.1016/0925-4773(93)90072-6

PMID:8494768

Abstract

The Drosophila CNS midline cells comprise a small, well-characterized group of neurons and glia in which the transcriptional control of CNS development can be studied. Using germ-line transformation of lacZ fusion constructs, we have dissected putative regulatory regions of the slit and Toll genes to identify CNS midline-restricted transcriptional enhancers. This analysis has uncovered DNA regions able to drive lacZ expression in most tissues in which embryonic slit and Toll are expressed, including three separable CNS midline-conferring regions: one in the Toll gene which is expressed early in all of the CNS midline precursors, and two in the slit gene which are expressed later in the midline glia (MG).

摘要

果蝇中枢神经系统中线细胞由一小群特征明确的神经元和神经胶质细胞组成，可用于研究中枢神经系统发育的转录调控。通过对lacZ融合构建体进行种系转化，我们剖析了slit和Toll基因的假定调控区域，以鉴定中枢神经系统中线特异性转录增强子。该分析揭示了能够在胚胎期slit和Toll表达的大多数组织中驱动lacZ表达的DNA区域，包括三个可分离的赋予中枢神经系统中线特性的区域：一个在Toll基因中，在所有中枢神经系统中线前体细胞中早期表达；另外两个在slit基因中，在中线神经胶质细胞（MG）中较晚表达。