Rosenblatt J E, Sloan L M, Schneider S K
Mayo Clinic, Rochester, MN 55905.
Diagn Microbiol Infect Dis. 1993 May-Jun;16(4):337-41. doi: 10.1016/0732-8893(93)90086-m.
A commercially available enzyme-linked immunosorbent assay (ELISA) for the detection of Giardia lamblia in stool specimens was evaluated on 342 specimens submitted to the Mayo Clinic Parasitology Laboratory for routine examination. The stools were either fresh or fresh/frozen at -65 degrees C (139 specimens) or were preserved in formalin (203 specimens). ELISA results were compared with those obtained by conventional microscopic examination: 143 stools were positive by both methods and 186 were negative. Sixty-six of the negative specimens contained 96 parasites belonging to 16 species other than Giardia, indicating a low rate of cross-reactivity. There were eight "false positive" ELISA results, which included specimens from one patient who had previously had a "true positive" and another patient with multiple family members infected with Giardia. Five stools that were "falsely negative" by ELISA contained only rare G. lamblia. The ELISA sensitivity was 97%, the specificity was 96%, and the positive predictive value was 95%. Results were evenly distributed between frozen and formalinized stools. The LMD/Seradyn ELISA appears to be a simple, rapid, and accurate method for the detection of G. lamblia in unprocessed stool samples.
在提交至梅奥诊所寄生虫学实验室进行常规检查的342份粪便标本上，对一种市售的用于检测粪便标本中蓝氏贾第鞭毛虫的酶联免疫吸附测定（ELISA）进行了评估。粪便标本要么是新鲜的，要么是在-65摄氏度下新鲜/冷冻保存的（139份标本），要么是保存在福尔马林中的（203份标本）。将ELISA检测结果与传统显微镜检查结果进行比较：两种方法均呈阳性的粪便标本有143份，呈阴性的有186份。66份阴性标本中含有96条属于除蓝氏贾第鞭毛虫之外16个物种的寄生虫，表明交叉反应率较低。有8份ELISA检测结果为“假阳性”，其中包括一名此前曾出现“真阳性”的患者的标本，以及另一名有多名家庭成员感染蓝氏贾第鞭毛虫的患者的标本。5份ELISA检测为“假阴性”的粪便标本中仅含有极少的蓝氏贾第鞭毛虫。ELISA的灵敏度为97%，特异性为96%，阳性预测值为95%。结果在冷冻粪便标本和经福尔马林固定的粪便标本之间均匀分布。LMD/Seradyn ELISA似乎是一种用于检测未经处理粪便样本中蓝氏贾第鞭毛虫的简单、快速且准确的方法。
