使用乳胶凝集-积分球比浊法（ISTA）快速检测血清乙肝表面抗体水平。

Quick assay of serum HBs antibody levels using latex agglutination-integrating sphere turbidimetric assay (ISTA).

作者信息

Yamashiki M, Kosaka Y, Nishimura A, Kondo I, Tomita A, Kishioka H

机构信息

Department of Laboratory Medicine, Mie University School of Medicine, Tokyo, Japan.

出版信息

J Clin Lab Anal. 1993;7(2):123-9. doi: 10.1002/jcla.1860070211.

DOI:10.1002/jcla.1860070211

PMID:8505697

Abstract

A method for determining serum HBs antibody applying the principle of integrating sphere turbidimetric assay (ISTA) by latex agglutination was developed. The minimum detectable level of HBs antibody by this method is 12.5 IU/L, indicating that this method is 3 times or more sensitive with better reproducibility and specificity than the passive hemagglutination (PHA) method. With a cut-off level of 25 IU/L, the possible highest simultaneous reading by this method was 1,000 IU/L. Serum HBs antibody can be readily measured in 10 or so minutes by this method if a fully automated EL-1000 analyzer is used. This rapid and simple method for determining serum HBs antibody will be useful not only clinically, but also in preventive medicine.

摘要

建立了一种基于乳胶凝集的积分球比浊法（ISTA）原理检测血清乙肝表面抗体（HBs抗体）的方法。该方法检测HBs抗体的最低检测限为12.5 IU/L，表明该方法比被动血凝试验（PHA）法灵敏3倍或更高，具有更好的重复性和特异性。以25 IU/L为临界值，该方法可能的最高同时读数为1000 IU/L。如果使用全自动EL-1000分析仪，用该方法大约10分钟就能轻松检测出血清HBs抗体。这种快速简便的血清HBs抗体检测方法不仅在临床上有用，在预防医学中也将发挥作用。