Bender E, Woof J M, Atkin J D, Barker M D, Bebbington C R, Burton D R
Krebs Institute, Department of Molecular Biology, University of Sheffield, UK.
Hum Antibodies Hybridomas. 1993 Apr;4(2):74-9.
The combinatorial phage library approach to immunoglobulin repertoire cloning recently made it possible to isolate gene fragments encoding human immunoglobulin G1 Fabs binding with high affinity to specific antigens. Here we describe the construction of genes encoding whole human anti-tetanus toxoid antibodies based on one of these gene fragments and the efficient expression of these constructs by co-transfection of separate heavy and light chain vectors into a Chinese hamster ovary cell line constitutively expressing a viral transactivator protein. This system will be generally useful for the rapid analysis of recombinant antibodies derived from combinatorial libraries.
组合噬菌体文库方法用于免疫球蛋白库克隆，最近使得分离编码与特定抗原具有高亲和力结合的人免疫球蛋白G1 Fab片段的基因片段成为可能。在此，我们描述了基于这些基因片段之一构建编码完整人抗破伤风类毒素抗体的基因，并通过将单独的重链和轻链载体共转染到组成型表达病毒反式激活蛋白的中国仓鼠卵巢细胞系中，实现这些构建体的高效表达。该系统对于快速分析来自组合文库的重组抗体将具有普遍用途。
