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酶促DNA还原的多样性:一种从马铃薯块茎中纯化DHA还原蛋白的新方法。

The multiplicity of enzymatic DNA reduction: a new purification procedure for a DHA reducing protein from potato tubers.

作者信息

De Tullio M C, Paciolla C, de Pinto M C, De Gara L

机构信息

Istituto di Botanica, Università di Bari.

出版信息

Boll Soc Ital Biol Sper. 1995 May-Jun;71(5-6):163-70.

PMID:8519491
Abstract

The multiplicity of DHA reducing proteins has been observed by means of a native-PAGE technique in several plant species, thus demonstrating, in accordance with recent literature, that several differently evolved proteins are likely to perform DHA reduction. Moreover, a research strategy coupling the use of native-PAGE with chromatographic separation procedure, tentatively performed in Solanum tuberosum, proved to be a useful tool for the separation and partial identification of the proteins involved in DHA reduction.

摘要

通过天然聚丙烯酰胺凝胶电泳技术,在几种植物物种中观察到了具有DHA还原功能的多种蛋白质,从而与最近的文献一致地证明,几种进化方式不同的蛋白质可能具有DHA还原功能。此外,在马铃薯中初步尝试的一种将天然聚丙烯酰胺凝胶电泳与色谱分离程序相结合的研究策略,被证明是分离和部分鉴定参与DHA还原的蛋白质的有用工具。

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