使用气相色谱-质谱联用技术鉴定人尿液中的9,10-环氧十八烷酸。

Identification of 9,10-epoxyoctadecanoic acid in human urine using gas chromatography-mass spectrometry.

作者信息

Ulsaker G A, Teien G

机构信息

Norwegian Medicines Control Authority, Oslo, Norway.

出版信息

Biomed Chromatogr. 1995 Jul-Aug;9(4):183-7. doi: 10.1002/bmc.1130090407.

DOI:10.1002/bmc.1130090407

PMID:8520208

Abstract

9,10-epoxyoctadecanoic acid has been detected in human urine. Two simple purification procedures were used; the one based upon liquid-liquid extraction and the other based upon sorbent extraction technology isolating the free fatty acid fraction. Prior to trimethylsilylation and gas chromatographic-mass spectrometric analysis, both the epoxy and carboxy functions were reduced to hydroxy groups. The shift in fragmentation of a deuterated sample verified the presence of intact epoxide prior to chemical reduction. Special attention was paid to the risk of false identification of the epoxide. The content of 9,10-epoxyoctadecanoic acid in human urine was estimated to be 2.1 nM/L.

摘要

在人尿中检测到了9,10-环氧十八烷酸。使用了两种简单的纯化程序；一种基于液-液萃取，另一种基于吸附剂萃取技术来分离游离脂肪酸部分。在进行三甲基硅烷化和气相色谱-质谱分析之前，环氧基和羧基官能团均被还原为羟基。氘代样品的碎片模式变化证实了化学还原前完整环氧化物的存在。特别关注了环氧化物错误鉴定的风险。估计人尿中9,10-环氧十八烷酸的含量为2.1 nM/L。