Kinne R W, Becker W, Koscheck T, Kuhlmann J, Sharkey R M, Behr T, Palombo-Kinne E, Goldenberg D M, Wolf F, Emmrich F
Max-Planck-Society, Department of Medicine III, University of Erlangen-Nuremberg, Germany.
J Nucl Med. 1995 Dec;36(12):2268-75.
The abundance of CD4 molecules on inflammatory cells in the synovial membrane renders anti-CD4 monoclonal antibodies (MAbs) or their fragments very promising for specific imaging of arthritic joints.
Joint uptake and body distribution of a 99mTc-labeled Fab', derived from the anti-rat CD4 MAb W3/25 (IgG1), were investigated following intravenous injection in normal and adjuvant arthritic rats. An isotype-matched Fab' (anti-human nonspecific crossreacting antigen-90) was used as control.
A 14-hr sequential pinhole scan of the ankle joints revealed that both the anti-CD4 and the control Fab' accumulated to a higher degree in arthritic than in normal ankle joints; however, accumulation of the anti-CD4 Fab' in arthritic joints exceeded that of the control Fab' (approximate to 1.6 fold). Preferential joint accumulation of anti-CD4 Fab' was confirmed by whole-body scans at 14 hr and by direct well counter measurements of tissue samples at 16 hr following injection. Unlike the control Fab', the anti-CD4 Fab' preferentially accumulated in the liver and lymph nodes, organs rich in CD4-positive cells, as observed by direct tissue measurements.
Despite its monovalency, the anti-CD4 Fab' retains the in vivo selectivity for CD4-positive cell-rich tissues, previously reported for the complete anti-CD4 MAb, and improves imaging of inflamed joints in experimental adjuvant arthritis.
滑膜中炎性细胞上CD4分子的丰度使得抗CD4单克隆抗体（MAb）或其片段在关节炎关节的特异性成像方面非常有前景。
在正常和佐剂性关节炎大鼠静脉注射后，研究了源自抗大鼠CD4单克隆抗体W3/25（IgG1）的99mTc标记的Fab′在关节的摄取和全身分布情况。使用同型匹配的Fab′（抗人非特异性交叉反应抗原-90）作为对照。
对踝关节进行14小时的连续针孔扫描显示，抗CD4和对照Fab′在关节炎关节中的积聚程度均高于正常踝关节；然而，抗CD4 Fab′在关节炎关节中的积聚超过了对照Fab′（约1.6倍）。注射后14小时的全身扫描以及16小时对组织样本的直接井型计数器测量证实了抗CD4 Fab′在关节中的优先积聚。与对照Fab′不同，通过直接组织测量观察到，抗CD4 Fab′优先积聚在肝脏和淋巴结中，这些器官富含CD4阳性细胞。
尽管抗CD4 Fab′具有单价性，但它保留了先前报道的完整抗CD4单克隆抗体对富含CD4阳性细胞组织的体内选择性，并改善了实验性佐剂性关节炎中炎症关节的成像。
