Guasch J F, Enfedaque J, Ferrer S, Gargallo D, Regué M
Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Barcelona, Spain.
Res Microbiol. 1995 Jul-Aug;146(6):477-83. doi: 10.1016/0923-2508(96)80293-2.
Twenty-six Serratia marcescens strains belonging to fifteen different biotypes were found to produce bacteriocins active against Escherichia coli. On the basis of the pattern of bacteriocin sensitivity of E. coli mutant strains, immunological assays and Southern blot hybridization with a probe for the S. marcescens bss (bacteriocin 28b structural) gene, it was concluded that all these strains apparently produce chromosomally encoded bacteriocins related to bacteriocin 28b. To confirm this conclusion, the genes encoding the bacteriocin produced by one of these strains (S. marcescens JF246) were cloned in plasmid pBR328. E. coli harbouring recombinant plasmid pDG301 produced a bacteriocin active against E. coli and immunologically related to bacteriocin 28b. Immunoblotting experiments showed that bacteriocins 28b and L appear to have the same apparent molecular mass (45 kDa). Furthermore, recombinant plasmid pDG301 DNA hybridized with a bss gene probe.
发现属于15种不同生物型的26株粘质沙雷氏菌产生对大肠杆菌有活性的细菌素。基于大肠杆菌突变株的细菌素敏感性模式、免疫测定以及用粘质沙雷氏菌bss（细菌素28b结构）基因探针进行的Southern印迹杂交，得出结论：所有这些菌株显然产生与细菌素28b相关的染色体编码细菌素。为证实这一结论，将其中一株菌株（粘质沙雷氏菌JF246）产生的细菌素的基因克隆到质粒pBR328中。携带重组质粒pDG301的大肠杆菌产生一种对大肠杆菌有活性且在免疫上与细菌素28b相关的细菌素。免疫印迹实验表明，细菌素28b和L似乎具有相同的表观分子量（45 kDa）。此外，重组质粒pDG301 DNA与bss基因探针杂交。
