四种新型的广宿主范围克隆载体pBBR1MCS的衍生物，携带不同的抗生素抗性盒。

Four new derivatives of the broad-host-range cloning vector pBBR1MCS, carrying different antibiotic-resistance cassettes.

作者信息

Kovach M E, Elzer P H, Hill D S, Robertson G T, Farris M A, Roop R M, Peterson K M

机构信息

Department of Microbiology and Immunology, Louisiana State University Medical Center-Shreveport 71130-3932, USA.

出版信息

Gene. 1995 Dec 1;166(1):175-6. doi: 10.1016/0378-1119(95)00584-1.

DOI:10.1016/0378-1119(95)00584-1

PMID:8529885

Abstract

Four new antibiotic-resistant derivatives of the broad-host-range (bhr) cloning vector pBBR1MCS have been constructed. These new plasmids have several advantages over many of the currently available bhr vectors in that: (i) they are relatively small (< 5.3 kb), (ii) they possess an extended multiple cloning site (MCS), (iii) they allow direct selection of recombinant plasmid molecules in Escherichia coli via disruption of the LacZ alpha peptide, (iv) they are mobilizable when the RK2 transfer functions are provided in trans and (v) they are compatible with IncP, IncQ and IncW group plasmids, as well as with ColE1- and P15a-based replicons.

摘要

已构建了广宿主范围（bhr）克隆载体pBBR1MCS的四种新的抗抗生素衍生物。这些新质粒相对于许多现有的bhr载体具有几个优点，即：（i）它们相对较小（<5.3 kb），（ii）它们具有扩展的多克隆位点（MCS），（iii）它们允许通过破坏LacZα肽在大肠杆菌中直接选择重组质粒分子，（iv）当反式提供RK2转移功能时它们是可移动的，以及（v）它们与IncP、IncQ和IncW组质粒以及与基于ColE1和P15a的复制子兼容。