Kayano K, Sakaida I, Kubota M, Yasunaga M, Okita K
First Department of Internal Medicine, Yamaguchi University, School of Medicine, Japan.
Liver. 1995 Oct;15(5):253-9. doi: 10.1111/j.1600-0676.1995.tb00681.x.
Activated liver macrophages are considered to play an important role in the development of liver injuries. Functional differences between activated and normal rat liver macrophages were investigated. In addition, from the therapeutic point of view, the effects of prostaglandin E1, prostaglandin I2 and E3330 ((2E)-3-[5-(2,3-dimethoxy-6-methyl-1,4- benzoquinoyl)]-2-nonyl-2-propenoic acid) on the functions of liver macrophages were also determined. Rat liver macrophages were primed by Propionibacterium acnes and activated by a small dose of lipopolysaccharide. Lipopolysaccharide uptake capacity was evaluated quantitatively by flow cytometric analysis. Tumor necrosis factor-alpha activities were measured by bioassay. There were no significant differences in lipopolysaccharide uptake capacity between activated and normal liver macrophages, while activated liver macrophages had a significantly (P < 0.01) higher capacity in the release of tumor necrosis factor-alpha. Prostaglandin E1 and E3330 inhibited tumor necrosis factor-alpha release without suppressing lipopolysaccharide uptake capacity. In this study we have clarified the functional differences between activated and normal liver macrophages. The beneficial effects of prostaglandin E1 and E3330 on the functions of liver macrophages were also demonstrated.
活化的肝巨噬细胞被认为在肝损伤的发展中起重要作用。研究了活化的大鼠肝巨噬细胞与正常大鼠肝巨噬细胞之间的功能差异。此外，从治疗角度出发，还测定了前列腺素E1、前列腺素I2和E3330（(2E)-3-[5-(2,3-二甲氧基-6-甲基-1,4-苯醌基)]-2-壬基-2-丙烯酸）对肝巨噬细胞功能的影响。大鼠肝巨噬细胞用痤疮丙酸杆菌预处理，并用小剂量脂多糖激活。通过流式细胞术分析定量评估脂多糖摄取能力。通过生物测定法测量肿瘤坏死因子-α活性。活化的肝巨噬细胞与正常肝巨噬细胞在脂多糖摄取能力上无显著差异，而活化的肝巨噬细胞在肿瘤坏死因子-α释放方面具有显著更高的能力（P < 0.01）。前列腺素E1和E3330抑制肿瘤坏死因子-α释放，而不抑制脂多糖摄取能力。在本研究中，我们阐明了活化的肝巨噬细胞与正常肝巨噬细胞之间的功能差异。还证明了前列腺素E1和E3330对肝巨噬细胞功能的有益作用。
