Ward P E, Russell J S, Vaghy P L
Department of Physiology, Ohio State University, Columbus 43210, USA.
Peptides. 1995;16(6):1073-8. doi: 10.1016/0196-9781(95)00082-u.
We have identified angiotensin-converting enzyme, neutral endopeptidase-24.11, and aminopeptidase M in a purified glycoprotein fraction of rabbit skeletal muscle membranes. The identification was based on substrate specificity and sensitivity to selective inhibitors. Angiotensin I metabolism was due to angiotensin-converting enzyme-mediated conversion to angiotensin II and neutral endopeptidase-24.11-mediated conversion to angiotensin(1-7). Bradykinin was degraded by angiotensin-converting enzyme and neutral endopeptidase-24.11; angiotensin II by neutral endopeptidase-24.11; and angiotensin III by neutral endopeptidase-24.11 and aminopeptidase M. Thus, the effects of angiotensins and kinins on skeletal muscle blood flow and metabolism may be regulated by local angiotensin-converting enzyme, neutral endopeptidase-24.11, and aminopeptidase M.
我们已在兔骨骼肌膜的纯化糖蛋白组分中鉴定出血管紧张素转换酶、中性内肽酶-24.11和氨肽酶M。该鉴定基于底物特异性和对选择性抑制剂的敏感性。血管紧张素I的代谢是由于血管紧张素转换酶介导其转化为血管紧张素II以及中性内肽酶-24.11介导其转化为血管紧张素(1-7)。缓激肽被血管紧张素转换酶和中性内肽酶-24.11降解；血管紧张素II被中性内肽酶-24.11降解；血管紧张素III被中性内肽酶-24.11和氨肽酶M降解。因此，血管紧张素和激肽对骨骼肌血流及代谢的影响可能受局部血管紧张素转换酶、中性内肽酶-24.11和氨肽酶M的调节。
