神经介素B刺激花生四烯酸释放、c-fos基因表达以及C6胶质瘤细胞的生长。

Neuromedin B stimulates arachidonic acid release, c-fos gene expression, and the growth of C6 glioma cells.

作者信息

Moody T W, Fagarasan M, Zia F

机构信息

National Cancer Institute, Biomarkers and Prevention Research Branch, Rockville, MD 20850, USA.

出版信息

Peptides. 1995;16(6):1133-40. doi: 10.1016/0196-9781(95)00085-x.

DOI:10.1016/0196-9781(95)00085-x

PMID:8532598

Abstract

The effects of neuromedin B (NMB) on C6 glioma cells were investigated. NMB bound with high affinity (IC50 = 1 nM) to C6 cells whereas BN and GRP were less potent (IC50 = 40 and 100 nM). NMB (1 nM) elevated cytosolic Ca2+ in individual C6 cells and the increase in cytosolic Ca2+ was reversed by 1 microM [D-Arg1, D-Pro2,D-Trp7.9,Leu11]substance P [APTTL]SP, a broad spectrum antagonist. NMB stimulated [3H]arachidonic acid release from C6 cells and the increase in [3H]arachidonic acid release was reversed [APTTL]SP. NMB increased transiently c-fos gene expression in C6 cells. NMB increased the number of C6 colonies in soft agar and the increase in growth caused by NMB was reversed by [APTTL]SP. These data suggest that NMB receptors may regulate the proliferation of C6 cells.

摘要

研究了神经介素B（NMB）对C6胶质瘤细胞的作用。NMB以高亲和力（IC50 = 1 nM）与C6细胞结合，而BN和GRP的效力较低（IC50 = 40和100 nM）。NMB（1 nM）可提高单个C6细胞的胞质Ca2+水平，1 microM [D-Arg1, D-Pro2,D-Trp7.9,Leu11]P物质[APTTL]SP（一种广谱拮抗剂）可逆转胞质Ca2+的升高。NMB刺激C6细胞释放[3H]花生四烯酸，[APTTL]SP可逆转[3H]花生四烯酸释放的增加。NMB可短暂增加C6细胞中c-fos基因的表达。NMB增加了软琼脂中C6集落的数量，[APTTL]SP可逆转NMB引起的生长增加。这些数据表明，NMB受体可能调节C6细胞的增殖。