通过克隆到大肠杆菌中的肺炎克雷伯菌4-羟基苯乙酸羟化酶基因进行聚合物生产。
Polymer production by Klebsiella pneumoniae 4-hydroxyphenylacetic acid hydroxylase genes cloned in Escherichia coli.
作者信息
Gibello A, Ferrer E, Sanz J, Martin M
机构信息
Departamento de Microbiología (Patología Animal I), Facultad de Veterinaria, Universidad Complutense, Madrid, Spain.
出版信息
Appl Environ Microbiol. 1995 Dec;61(12):4167-71. doi: 10.1128/aem.61.12.4167-4171.1995.
Abstract
The expression of Klebsiella pneumoniae hpaA and hpaH genes, which code for 4-hydroxyphenylacetic acid hydroxylase in Escherichia coli K-12 derivative strains, is associated with the production of a dark brown pigment in the cultures. This pigment has been identified as a polymer which shows several of the characteristics reported for microbial melanins and results from the oxidative activity of 4-hydroxyphenylacetic acid hydroxylase on some dihydroxylated compounds to form o-quinones. A dibenzoquinone is formed from the oxidation of different mono- or dihydroxylated aromatic compounds by the enzyme prior to polymerization. We report a hydroxylase activity, other than tyrosinase, that is associated with the synthesis of a bacterial melanin.
摘要
肺炎克雷伯菌hpaA和hpaH基因在大肠杆菌K-12衍生菌株中编码4-羟基苯乙酸羟化酶，其表达与培养物中深棕色色素的产生有关。这种色素已被鉴定为一种聚合物，它具有一些已报道的微生物黑色素的特征，是4-羟基苯乙酸羟化酶对一些二羟基化化合物进行氧化活性以形成邻醌的结果。在聚合之前，该酶通过氧化不同的单羟基或二羟基化芳香化合物形成二苯醌。我们报道了一种除酪氨酸酶外与细菌黑色素合成相关的羟化酶活性。
相似文献
1
Polymer production by Klebsiella pneumoniae 4-hydroxyphenylacetic acid hydroxylase genes cloned in Escherichia coli.通过克隆到大肠杆菌中的肺炎克雷伯菌4-羟基苯乙酸羟化酶基因进行聚合物生产。
Appl Environ Microbiol. 1995 Dec;61(12):4167-71. doi: 10.1128/aem.61.12.4167-4171.1995.
2
Molecular cloning and analysis of the genes encoding the 4-hydroxyphenylacetate hydroxylase from Klebsiella pneumoniae.肺炎克雷伯菌4-羟基苯乙酸羟化酶编码基因的分子克隆与分析
Arch Microbiol. 1997 Feb-Mar;167(2-3):160-6.
3
Catabolism of 3- and 4-hydroxyphenylacetic acid by Klebsiella pneumoniae.肺炎克雷伯菌对3-和4-羟基苯乙酸的分解代谢
J Gen Microbiol. 1991 Mar;137(3):621-8. doi: 10.1099/00221287-137-3-621.
4
Molecular characterization of the 4-hydroxyphenylacetate catabolic pathway of Escherichia coli W: engineering a mobile aromatic degradative cluster.大肠杆菌W的4-羟基苯乙酸分解代谢途径的分子特征：构建一个可移动的芳香族降解基因簇
J Bacteriol. 1996 Jan;178(1):111-20. doi: 10.1128/jb.178.1.111-120.1996.
5
Characterization of an Escherichia coli aromatic hydroxylase with a broad substrate range.具有广泛底物范围的大肠杆菌芳香羟化酶的特性研究
J Bacteriol. 1993 Apr;175(7):2162-7. doi: 10.1128/jb.175.7.2162-2167.1993.
6
Purification and physiochemical characterization of melanin pigment from Klebsiella sp. GSK.从 Klebsiella sp. GSK 中纯化和生理化学特性表征黑色素色素。
J Microbiol Biotechnol. 2010 Nov;20(11):1513-20. doi: 10.4014/jmb.1002.02006.
7
Multidrug resistance in Klebsiella pneumoniae: a novel gene, ramA, confers a multidrug resistance phenotype in Escherichia coli.肺炎克雷伯菌中的多重耐药性：一个新基因ramA赋予大肠杆菌多重耐药表型。
Microbiology (Reading). 1995 Aug;141 ( Pt 8):1909-1920. doi: 10.1099/13500872-141-8-1909.
8
Occurrence and regulation of the ferric citrate transport system in Escherichia coli B, Klebsiella pneumoniae, Enterobacter aerogenes, and Photorhabdus luminescens.大肠杆菌B、肺炎克雷伯菌、产气肠杆菌和发光光杆状菌中柠檬酸铁转运系统的发生与调控。
Arch Microbiol. 2005 Nov;184(3):175-86. doi: 10.1007/s00203-005-0035-y. Epub 2005 Nov 10.
9
Cloning, sequencing and characterization of Fnr from Klebsiella pneumoniae.肺炎克雷伯菌Fnr的克隆、测序及特性分析
Antonie Van Leeuwenhoek. 2001 Sep;79(3-4):319-26. doi: 10.1023/a:1012060730647.
10
Biosynthesis of Klebsiella K2 capsular polysaccharide in Escherichia coli HB101 requires the functions of rmpA and the chromosomal cps gene cluster of the virulent strain Klebsiella pneumoniae Chedid (O1:K2).肺炎克雷伯菌Chedid（O1:K2）强毒株的rmpA和染色体cps基因簇功能是大肠杆菌HB101中克雷伯菌K2荚膜多糖生物合成所必需的。
Infect Immun. 1991 Jun;59(6):2043-50. doi: 10.1128/iai.59.6.2043-2050.1991.
引用本文的文献
1
4-Hydroxyphenylacetate 3-Hydroxylase (4HPA3H): A Vigorous Monooxygenase for Versatile -Hydroxylation Applications in the Biosynthesis of Phenolic Derivatives.4-羟基苯乙酸3-羟化酶（4HPA3H）：一种用于酚类衍生物生物合成中多种β-羟化应用的高效单加氧酶。
Int J Mol Sci. 2024 Jan 19;25(2):1222. doi: 10.3390/ijms25021222.
2
New insights and advances on pyomelanin production: from microbial synthesis to applications.关于脓黑素产生的新见解和进展：从微生物合成到应用。
J Ind Microbiol Biotechnol. 2022 Jul 30;49(4). doi: 10.1093/jimb/kuac013.
3
Production of Melanins With Recombinant Microorganisms.利用重组微生物生产黑色素
Front Bioeng Biotechnol. 2019 Oct 24;7:285. doi: 10.3389/fbioe.2019.00285. eCollection 2019.
4
Activation of Melanin Synthesis in Alternaria infectoria by Antifungal Drugs.抗真菌药物对链格孢菌黑色素合成的激活作用。
Antimicrob Agents Chemother. 2015 Dec 28;60(3):1646-55. doi: 10.1128/AAC.02190-15.
5
Cloning and characterization of a 4-hydroxyphenylacetate 3-hydroxylase from the thermophile Geobacillus sp. PA-9.
Curr Microbiol. 2007 Dec;55(6):480-4. doi: 10.1007/s00284-007-9016-5. Epub 2007 Sep 5.
6
Induction by Klebsiella aerogenes of a melanin-like pigment in Cryptococcus neoformans.产气克雷伯菌对新型隐球菌中一种黑色素样色素的诱导作用。
Appl Environ Microbiol. 2006 Feb;72(2):1542-50. doi: 10.1128/AEM.72.2.1542-1550.2006.
7
Biodegradation of aromatic compounds by Escherichia coli.大肠杆菌对芳香族化合物的生物降解作用。
Microbiol Mol Biol Rev. 2001 Dec;65(4):523-69, table of contents. doi: 10.1128/MMBR.65.4.523-569.2001.
8
Characterization of 4-hydroxyphenylacetate 3-hydroxylase (HpaB) of Escherichia coli as a reduced flavin adenine dinucleotide-utilizing monooxygenase.大肠杆菌4-羟基苯乙酸3-羟化酶（HpaB）作为利用还原型黄素腺嘌呤二核苷酸的单加氧酶的特性分析。
Appl Environ Microbiol. 2000 Feb;66(2):481-6. doi: 10.1128/AEM.66.2.481-486.2000.
9
Genes for 2,4,5-trichlorophenoxyacetic acid metabolism in Burkholderia cepacia AC1100: characterization of the tftC and tftD genes and locations of the tft operons on multiple replicons.洋葱伯克霍尔德菌AC1100中2,4,5-三氯苯氧乙酸代谢相关基因：tftC和tftD基因的特征以及tft操纵子在多个复制子上的定位
Appl Environ Microbiol. 1998 Jun;64(6):2086-93. doi: 10.1128/AEM.64.6.2086-2093.1998.
本文引用的文献
1
Isolation and characterization of melanin-producing (mel) mutants of Vibrio cholerae.霍乱弧菌产黑色素（mel）突变体的分离与鉴定
Infect Immun. 1980 Mar;27(3):721-9. doi: 10.1128/iai.27.3.721-729.1980.
2
Enzymology of oxygen.氧的酶学
Annu Rev Biochem. 1982;51:21-59. doi: 10.1146/annurev.bi.51.070182.000321.
3
The production and some properties of the brown pigment of Aeromonas liquefaciens.液化气单胞菌棕色色素的产生及某些特性
Acta Vet Scand. 1972;13(2):251-9. doi: 10.1186/BF03548579.
4
Pedigrees of some mutant strains of Escherichia coli K-12.大肠杆菌K-12某些突变菌株的谱系。
Bacteriol Rev. 1972 Dec;36(4):525-57. doi: 10.1128/br.36.4.525-557.1972.
5
The metabolism of phenylacetic acid by a Pseudomonas.一种假单胞菌对苯乙酸的代谢
Can J Microbiol. 1967 Feb;13(2):147-57. doi: 10.1139/m67-021.
6
Purification and properties of tyrosinases from Vibrio tyrosinaticus.来自酪氨酸弧菌的酪氨酸酶的纯化及性质
Arch Biochem Biophys. 1974 Jan;160(1):73-82. doi: 10.1016/s0003-9861(74)80010-x.
7
Extracellular tyrosinase from Streptomyces sp. KY-453: purification and some enzymatic properties.链霉菌属KY-453的胞外酪氨酸酶：纯化及一些酶学性质
J Biochem. 1985 Jun;97(6):1747-54. doi: 10.1093/oxfordjournals.jbchem.a135233.
8
Dechlorination and para-hydroxylation of polychlorinated phenols by Rhodococcus chlorophenolicus.氯酚红球菌对多氯酚的脱氯及对羟基化作用
J Bacteriol. 1987 Feb;169(2):675-81. doi: 10.1128/jb.169.2.675-681.1987.
9
Cyst production and brown pigment formation in aging cultures of Azospirillum brasilense ATCC 29145.巴西固氮螺菌ATCC 29145衰老培养物中的孢囊产生和褐色色素形成。
J Bacteriol. 1987 Apr;169(4):1670-7. doi: 10.1128/jb.169.4.1670-1677.1987.
10
Initial catabolism of aromatic biogenic amines by Pseudomonas aeruginosa PAO: pathway description, mapping of mutations, and cloning of essential genes.铜绿假单胞菌PAO对芳香族生物胺的初始分解代谢：途径描述、突变定位及必需基因的克隆
J Bacteriol. 1987 Jun;169(6):2398-404. doi: 10.1128/jb.169.6.2398-2404.1987.
Zotero 插件