视紫红质的朗缪尔-布洛杰特膜的表征：热稳定性研究。
Characterization of Langmuir-Blodgett films of rhodopsin: thermal stability studies.
作者信息
Maxia L, Radicchi G, Pepe I M, Nicolini C
机构信息
Institute of Biophysics, Faculty of Medicine, University of Genova, Italy.
出版信息
Biophys J. 1995 Oct;69(4):1440-6. doi: 10.1016/S0006-3495(95)80013-5.
Two-dimensional close packing of purified bovine rhodopsin, made by the Langmuir-Blodgett technique, was characterized by small angle x-ray scattering and nanogravimetric measurements. The area occupied by a molecule of rhodopsin in the film was approximately 1100 Angstrum2 and the periodicity of the layers resulted in 59 Angstrum. The circular dichroism measurements showed that bleached rhodopsin in Langmuir-Blodgett film had high thermal stability, in fact, reaching a temperature of 150 degrees C without a loss of the secondary structure. Moreover, when the film was made up in the dark, rhodopsin maintained its stability up to at least 200 degrees C and its characteristic absorbance peak at 500 nm up to about 90 degrees C.
通过朗缪尔-布洛杰特技术制备的纯化牛视紫红质的二维紧密堆积，采用小角X射线散射和纳米重力测量进行表征。视紫红质分子在膜中占据的面积约为1100埃²，层的周期性导致间距为59埃。圆二色性测量表明，朗缪尔-布洛杰特膜中的漂白视紫红质具有高热稳定性，实际上，在二级结构不损失的情况下能达到150摄氏度。此外，当膜在黑暗中制备时，视紫红质在至少200摄氏度时仍保持其稳定性，其在500纳米处的特征吸收峰在约90摄氏度之前保持不变。
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