Kuspa A, Dingermann T, Nellen W
Department of Biochemistry, Baylor College of Medicine, Houston, Texas 77030, USA.
Experientia. 1995 Dec 18;51(12):1116-23. doi: 10.1007/BF01944729.
Over the past ten years, powerful molecular genetic techniques have been developed to analyze gene function in Dictyostelium. DNA-mediated transformation using a variety of selections and vectors has allowed the introduction of wild-type or modified genes that are under various forms of transcriptional control. Homologous recombination is efficient and can be used to modify the genome in precise ways. In addition, it is now possible to clone genes based on their mutant phenotype alone, either by insertional mutagenesis, or by screening antisense expression cDNA libraries. Finally, a nearly complete physical map of the genome is available and so genes are easily mapped by physical techniques. We discuss many of these advances within the context of major research problems presently under study.
在过去十年中，已开发出强大的分子遗传学技术来分析盘基网柄菌中的基因功能。使用各种选择方法和载体进行的DNA介导转化，使得能够引入处于各种转录控制形式下的野生型或修饰基因。同源重组效率很高，可用于以精确方式修饰基因组。此外，现在仅凭突变体表型就可以通过插入诱变或筛选反义表达cDNA文库来克隆基因。最后，基因组的近乎完整的物理图谱已经可用，因此可以通过物理技术轻松地对基因进行定位。我们将在目前正在研究的主要研究问题的背景下讨论其中的许多进展。
