水稻叶片铁氧还蛋白-亚硝酸还原酶cDNA的克隆与核苷酸序列分析

Cloning and nucleotide sequence of a leaf ferredoxin-nitrite reductase cDNA of rice.

作者信息

Terada Y, Aoki H, Tanaka T, Morikawa H, Ida S

机构信息

Research Institute for Food Science, Kyoto University, Japan.

出版信息

Biosci Biotechnol Biochem. 1995 Nov;59(11):2183-5. doi: 10.1271/bbb.59.2183.

DOI:10.1271/bbb.59.2183

PMID:8541663

Abstract

A ferredoxin-nitrite reductase (EC 1.7.7.1) cDNA was isolated and sequenced from a lambda gt 11 cDNA library constructed from nitrate-induced greening shoots of rice (Oryza sativa L.) seedlings. The nucleotide sequence of the cDNA clone contains an open reading frame of 1788 nucleotides. There exists a strong bias for the third codon usage of G/C (95.5%) as in the case of the maize enzyme. The deduced amino acid sequence shows an overall homology to the maize (81%) and the dicot enzymes (70-74%), suggesting that the primary structure of ferredoxin-nitrite reductase is highly conserved in higher plants.

摘要

从由水稻（Oryza sativa L.）幼苗经硝酸盐诱导变绿的茎构建的λgt 11 cDNA文库中分离并测序了一种铁氧化还原蛋白-亚硝酸盐还原酶（EC 1.7.7.1）cDNA。该cDNA克隆的核苷酸序列包含一个1788个核苷酸的开放阅读框。与玉米酶的情况一样，第三个密码子的使用存在强烈的G/C偏向性（95.5%）。推导的氨基酸序列与玉米酶（81%）和双子叶植物酶（70 - 74%）具有整体同源性，表明铁氧化还原蛋白-亚硝酸盐还原酶的一级结构在高等植物中高度保守。