与人类RCK/P54同源的小鼠cDNA的克隆与表达，RCK/P54是位于11q23的一个与淋巴瘤相关的染色体易位连接基因。
Cloning and expression of a murine cDNA homologous to the human RCK/P54, a lymphoma-linked chromosomal translocation junction gene on 11q23.
作者信息
Seto M, Yamamoto K, Takahashi T, Ueda R
机构信息
Laboratory of Chemotherapy, Aichi Cancer Center Research Institute, Nagoya, Japan.
出版信息
Gene. 1995 Dec 12;166(2):293-6. doi: 10.1016/0378-1119(95)00559-5.
The RCK/P54 gene was previously isolated from the chromosome translocation breakpoint region on 11q23 of a human lymphoma cell line, RC-K8, with t(11;14)(q23;q32). It was found to encode a 472-483-amino-acid (aa) polypeptide belonging to an RNA helicase/translation initiation factor family. The aim of the present investigation was the isolation and comparative sequence analysis of the mouse RCK/P54 cDNA from a BALB/c spleen cDNA library. Sequence analysis revealed an open reading frame (ORF) predicting a polypeptide of 483 aa showing 97.7% identity to human RCK/p54. Northern analysis demonstrated a 7.5-kb signal in all tissues tested and in vitro translation analysis showed a 54-kDa polypeptide. These results indicate that both mouse and human RCK/P54 are highly conserved.
RCK/P54基因先前是从一个人淋巴瘤细胞系RC-K8的11q23染色体易位断点区域分离出来的，该细胞系具有t(11;14)(q23;q32)。发现它编码一种属于RNA解旋酶/翻译起始因子家族的472 - 483个氨基酸（aa）的多肽。本研究的目的是从BALB/c脾cDNA文库中分离小鼠RCK/P54 cDNA并进行比较序列分析。序列分析揭示了一个开放阅读框（ORF），预测出一个483个氨基酸的多肽，与人RCK/p54的同一性为97.7%。Northern分析在所有测试组织中显示出一个7.5 kb的信号，体外翻译分析显示出一个54 kDa的多肽。这些结果表明小鼠和人RCK/P54都高度保守。
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