Soligo D, de Harven E P, Quirici N, Caneva L, Lambertenghi Deliliers G
Centro Trapianti di Midollo, Ospedale Maggiore di Milano, IRCCS, Italy.
Scanning Microsc. 1995 Mar;9(1):175-83; discussion 183-4.
Hemopoietic cells display a wide range of cell surface antigens which are either lineage specific or acquired during differentiation. Monoclonal antibodies can be used, in conjunction with colloidal gold markers, to identify under the scanning electron microscopy (SEM) at the single cell level, specific lineage or maturation stages in the hemopoietic bone marrow. Normal bone marrow cells, either gradient separated or purified by immuno-magnetic methods and leukemic cell samples, which can be considered as "frozen" stages of hemopoietic differentiation, have been studied with this method. Typical cell surface morphologies, which characterize immature progenitor cells and cells committed or differentiated towards the lymphoid, myeloid, erythroid and megakaryocytic lineage have been identified. Correlations between cell surface features and some hemopoietic cells functions have been attempted on the basis of these findings.
造血细胞呈现出多种细胞表面抗原，这些抗原要么是谱系特异性的，要么是在分化过程中获得的。单克隆抗体可与胶体金标记物结合使用，以便在扫描电子显微镜（SEM）下在单细胞水平识别造血骨髓中的特定谱系或成熟阶段。已使用此方法研究了通过梯度分离或免疫磁选法纯化的正常骨髓细胞以及白血病细胞样本，这些样本可被视为造血分化的“冻结”阶段。已识别出表征未成熟祖细胞以及向淋巴、髓系、红系和巨核细胞谱系定向或分化的细胞的典型细胞表面形态。基于这些发现，已尝试研究细胞表面特征与一些造血细胞功能之间的相关性。
