Lee S L, Wasserlauf H G, Brault J S, Freydl K R, Earman W A, Glonek T O
Department of Biochemistry, Midwestern University, Downers Grove, Illinois, USA.
Spine (Phila Pa 1976). 1995 Nov 1;20(21):2278-82. doi: 10.1097/00007632-199511000-00003.
31P Nuclear magnetic resonance spectroscopy was used to compare amounts and types of principal organophosphorus biomolecules in canine intervertebral disc tissues ex vivo.
The goal of this study was to compare principal organophosphorus biomolecules in nucleus pulposus, anulus fibrosus, and in isolated nucleus pulposus and anulus fibrosus cells.
There is no published information on principal organophosphorus biomolecules in disc tissues for any animal.
Canine nucleus pulposus and anulus fibrosus were shown by hematoxylin-eosin staining to be healthy tissues characteristic of adult dogs. Viable cells liberated from these disc tissues by sequential protease digestion were directly visualized by light microscopy of wet mounts. 31P nuclear magnetic resonance spectra were recorded at room temperature for 12 hours according to conventional published methods.
No resonances were detected for intact nucleus pulposus and anulus fibrosus tissue. 31P nuclear magnetic resonance spectra of viable chondrocyte-like cells from anulus fibrosus featured two distinctive resonance peaks consistent with phosphomonoesters and phosphodiesters. After undigested anulus fibrosus was removed, no resonances were detected. 31P nuclear magnetic resonance spectra of viable chondrocyte-like cells from nucleus pulposus before and after removal of undigested tissue featured only one resonance peak consistent with phosphodiesters.
A phosphomonoester-containing biomolecule is present in healthy canine anulus fibrosus tissue but not in nucleus pulposus tissue, nor in nucleus pulposus cells or anulus fibrosus cells. A phosphodiester-containing biomolecule is present principally in nucleus pulposus cells. This study demonstrated that canine chondrocyte-like cells from nucleus pulposus and anulus fibrosus are phenotypically distinctive in principal organophosphorus biomolecules.
采用31P核磁共振波谱法对犬椎间盘组织离体后的主要有机磷生物分子的数量和类型进行比较。
本研究旨在比较髓核、纤维环以及分离出的髓核细胞和纤维环细胞中的主要有机磷生物分子。
目前尚无关于任何动物椎间盘组织中主要有机磷生物分子的公开信息。
苏木精-伊红染色显示犬的髓核和纤维环为成年犬的健康组织特征。通过连续蛋白酶消化从这些椎间盘组织中释放出的活细胞，经湿片光学显微镜直接观察。按照传统公开方法在室温下记录31P核磁共振波谱12小时。
完整的髓核和纤维环组织未检测到共振信号。纤维环中存活的软骨样细胞的31P核磁共振波谱有两个与磷酸单酯和磷酸二酯一致的独特共振峰。去除未消化的纤维环后，未检测到共振信号。去除未消化组织前后髓核中存活的软骨样细胞的31P核磁共振波谱仅出现一个与磷酸二酯一致的共振峰。
健康犬的纤维环组织中存在含磷酸单酯的生物分子，但髓核组织、髓核细胞或纤维环细胞中不存在。含磷酸二酯的生物分子主要存在于髓核细胞中。本研究表明，犬髓核和纤维环中的软骨样细胞在主要有机磷生物分子方面具有表型差异。
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