Angelov D N, Neiss W F, Streppel M, Andermahr J, Mader K, Stennert E
Institut I für Anatomie, Universität zu Köln, Cologne, Germany.
J Neurosci. 1996 Feb 1;16(3):1041-8. doi: 10.1523/JNEUROSCI.16-03-01041.1996.
Facial-facial anastomosis (FFA), i.e., suture of transected facial nerve, was performed in adult Wistar rats. For 10-112 d post-operation (DPO), half of the animals received standard food (placebo) and half received food pellets containing 1000 ppm nimodipine, a Ca2+ channel blocker. The time course of mimetic reinnervation between these two groups was compared by counting all retrogradely labeled motoneurons after injection of horseradish peroxidase (HRP) into the whiskerpad. In unoperated animals, injection of HRP labeled 1280 +/- 113 motoneurons. After FFA, this number dropped to zero, and the first HRP-labeled facial motoneurons reappeared in both placebo- and nimodipine-treated animals at 14 DPO. The treatment with nimodipine yielded two beneficial effects. (1) It accelerated axonal sprouting until 28 DPO. Whereas the number of HRP-labeled cells in the placebo group was 171 +/- 9 (mean +/- SD) at 16 DPO, 372 +/- 43 at 21 DPO, and 636 +/- 187 at 28 DPO, the number of sprouted motoneurons in nimodipine-treated rats was twice as high: 386 +/- 34 at 16 DPO, 620 +/- 28 at 21 DPO, and 756 +/- 257 at 28 DPO. (2) Nimodipine reduced the polyneuronal innervation of the target muscles. Whereas the number of HRP-labeled cells in the placebo group increased to 1430 +/- 36 at 56 DPO and 1600 +/- 31 at 112 DPO, the number of labeled motoneurons in nimodipine-treated rats remained almost within the normal range: 1315 +/- 31 at 56 DPO and 1354 +/- 33 at 112 DPO.
在成年Wistar大鼠中进行了面神经-面神经吻合术（FFA），即切断面神经的缝合。术后10 - 112天（DPO），一半动物给予标准食物（安慰剂），另一半给予含1000 ppm尼莫地平（一种钙通道阻滞剂）的食丸。通过向触须垫注射辣根过氧化物酶（HRP）后计数所有逆行标记的运动神经元，比较这两组之间模拟再支配的时间进程。在未手术的动物中，注射HRP标记了1280±113个运动神经元。FFA后，这个数字降至零，并且在14 DPO时，安慰剂组和尼莫地平治疗组的动物中首次出现了HRP标记的面神经运动神经元。尼莫地平治疗产生了两个有益效果。（1）它加速了轴突发芽直至28 DPO。安慰剂组中HRP标记细胞的数量在16 DPO时为171±9（平均值±标准差），21 DPO时为372±43，28 DPO时为636±187，而尼莫地平治疗的大鼠中发芽运动神经元的数量是其两倍：16 DPO时为386±34，21 DPO时为620±28，28 DPO时为756±257。（2）尼莫地平减少了靶肌肉的多神经元支配。安慰剂组中HRP标记细胞的数量在56 DPO时增加到1430±36，在112 DPO时增加到1600±31，而尼莫地平治疗的大鼠中标记运动神经元的数量几乎保持在正常范围内：56 DPO时为1315±31，112 DPO时为1354±33。
