Dagenais F, Buluran J, Cartier R
Département de Chirurgie, Institut de Cardiologie de Montréal, Québec, Canada.
Ann Chir. 1995;49(8):700-5.
Optimal solution for endothelial function preservation during cold storage for organ transplant has not yet been defined. To assess this issue, rat aortic rings (n = 28) were stored for 4 hours at 4 degrees C in different preservation solutions: Krebs Ringer (KR), normal saline (NS), rat blood (RB), and the University of Wisconsin solution (UW). Subsequently, rings were suspended in organ chambers for endothelial and smooth muscle assessment. Endothelial-dependent relaxation responses were tested by exposure to cumulative doses of acetylcholine (ACH), adenosine diphosphate (ADP), and histamine (HIS). Smooth muscle function was evaluated by exposure to norepinephrine (NE) and sodium nitroprusside (SNP). A fifth group not submitted to a storage period was used as control (CTL, n = 7). Results are expressed as maximal relaxation (%) from initial precontraction level. Compared to the CTL group, all storage groups showed a significant decrease in endothelial-dependent relaxations to ACH, ADP and HIS (p < 0.05). Among the stored groups, endothelial-dependent relaxation to ACH was significantly decreased in the NS group when compared to the UW group (p < 0.05). Endothelial-independent relaxation to SNP was comparable for all groups. However significant hypercontractility to NE was observed for all stored groups compared to the control group (p < 0.01). A significant decrease in ADP response was observed with NS and RB storage when compared to KR (p < 0.01). In conclusion, cold storage affects both endothelial and smooth muscle function by decreasing endothelial-dependent responses to ACH, ADP and HIS and increasing smooth muscle reactivity to NE. However, endothelial dysfunction was lessened with UW or KR storage compared to NS or RB.
器官移植冷藏期间内皮功能保护的最佳解决方案尚未确定。为评估此问题，将28个大鼠主动脉环在4℃下于不同保存溶液中保存4小时： Krebs Ringer（KR）、生理盐水（NS）、大鼠血液（RB）和威斯康星大学溶液（UW）。随后，将主动脉环悬挂在器官腔室中进行内皮和平滑肌评估。通过暴露于累积剂量的乙酰胆碱（ACH）、二磷酸腺苷（ADP）和组胺（HIS）来测试内皮依赖性舒张反应。通过暴露于去甲肾上腺素（NE）和硝普钠（SNP）来评估平滑肌功能。将未经历保存期的第五组用作对照（CTL，n = 7）。结果以相对于初始预收缩水平的最大舒张率（%）表示。与CTL组相比，所有保存组对ACH、ADP和HIS的内皮依赖性舒张均显著降低（p < 0.05）。在保存组中，与UW组相比，NS组对ACH的内皮依赖性舒张显著降低（p < 0.05）。所有组对SNP的非内皮依赖性舒张相当。然而，与对照组相比，所有保存组均观察到对NE的显著高收缩性（p < 0.01）。与KR相比，NS和RB保存时观察到ADP反应显著降低（p < 0.01）。总之，冷藏通过降低对ACH、ADP和HIS的内皮依赖性反应以及增加平滑肌对NE的反应性来影响内皮和平滑肌功能。然而，与NS或RB相比，UW或KR保存时内皮功能障碍减轻。
