小鼠γ-谷氨酰转肽酶编码基因的cDNA克隆及基因组结构

Cloning of cDNA and genomic structure of the mouse gamma-glutamyl transpeptidase-encoding gene.

作者信息

Shi Z Z, Habib G M, Lebovitz R M, Lieberman M W

机构信息

Department of Pathology, Baylor College of Medicine, Houston, TX 77030, USA.

出版信息

Gene. 1995 Dec 29;167(1-2):233-7. doi: 10.1016/0378-1119(95)00618-4.

DOI:10.1016/0378-1119(95)00618-4

PMID:8566783

Abstract

We have isolated and characterized cDNA and genomic clones containing the coding region for the mouse gamma-glutamyl transpeptidase (GGT). The sequences of the full-length cDNAs for three of the seven known mouse Ggt RNAs (types I, II and III) were determined and found to be identical in the coding region. Comparisons of the deduced amino-acid sequence of mouse GGT with that of rat and human reveal 95 and 79% overall identities, respectively. The mouse Ggt gene has 12 coding exon and spans approx. 12 kb. We have also re-analyzed rat genomic Ggt clones previously isolated by us and found that the rat and mouse genes share the same intron/exon boundaries. Our findings are of interest because they define the structure of the mouse and rat Ggt genes and will allow comparison with human GGT genes which, recent findings suggest, have diverged substantially from rodents.

摘要

我们已经分离并鉴定了包含小鼠γ-谷氨酰转肽酶（GGT）编码区的cDNA和基因组克隆。测定了七种已知小鼠Ggt RNA中的三种（I型、II型和III型）全长cDNA的序列，发现它们在编码区是相同的。将小鼠GGT推导的氨基酸序列与大鼠和人类的进行比较，总体同一性分别为95%和79%。小鼠Ggt基因有12个编码外显子，跨度约为12 kb。我们还重新分析了我们之前分离的大鼠基因组Ggt克隆，发现大鼠和小鼠基因具有相同的内含子/外显子边界。我们的发现很有意义，因为它们定义了小鼠和大鼠Ggt基因的结构，并将有助于与人类GGT基因进行比较，最近的研究结果表明，人类GGT基因与啮齿动物的基因已经有了很大的差异。