Effects of atrial natriuretic factor on norepinephrine release evoked by angiotensins II and III in the rat adrenal medulla.

Atrial natriuretic factor (ANF) effects on neuronal norepinephrine (NE) release evoked by angiotensin II (ANG II) or angiotensin III (ANG III) were studied in the rat adrenal medulla. ANF 10 nM diminished the increase of NE release induced by ANG II (1 microM), ANG III (1 microM) or 100 mM KCl. When 10 nM ANF was added to the medium containing KCl plus ANG II or KCl plus ANG III, the reduction of 3H-NE output by ANF was greater than when the atrial factor was added to the medium containing only ANG II or ANG III. Since both ANG II and ANG III have a physiological role on catecholamine metabolism, these peptides could modulate the adrenal medulla functions. ANG II and ANG III enhance NE release and decrease NE uptake in the rat adrenal medulla. Present results show that ANF is a physiological antagonist of both ANG II and ANG III, in the process of NE secretion. The interaction between ANF and the renin-angiotensin system could contribute to the regulation of the adrenal medulla catecholamines pathway and sympathetic activity.