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天青B-伊红APAAP染色:一种同时进行血液学和免疫学细胞分析的方法。

Azure B-eosin APAAP staining: a method for simultaneous hematological and immunological cell analysis.

作者信息

Koch B, Eberhardt B, Westerhausen M

机构信息

Department of Internal Medicine, University of Homburg, Germany.

出版信息

Biotech Histochem. 1995 Sep;70(5):267-70. doi: 10.3109/10520299509108205.

Abstract

Azure B-eosin APAAP staining allows simultaneous analysis of peripheral blood and bone marrow cells for hematological characteristics and immunological cell marker profiles. A defined sequence of staining procedures maintains characteristic components of the Romanowsky-Giemsa stain whereas cell antigens can be detected immunologically using the alkaline phosphatase-anti-alkaline phosphatase (APAAP) detection system. Antigens are visualized by the staining product of the substrate-naphthol AS GR phosphate and variamine blue salt. The usefulness of the azure B-eosin APAAP method was demonstrated on blood and bone marrow smears of patients with various hematological disorders.

摘要

天青B-伊红碱性磷酸酶抗碱性磷酸酶(APAAP)染色可同时分析外周血和骨髓细胞的血液学特征及免疫细胞标志物谱。特定的染色程序序列可保留瑞氏-吉姆萨染色的特征成分,而细胞抗原可通过碱性磷酸酶抗碱性磷酸酶(APAAP)检测系统进行免疫检测。抗原通过底物-萘酚AS GR磷酸酯和变色胺蓝盐的染色产物显现。天青B-伊红APAAP方法在各种血液系统疾病患者的血液和骨髓涂片上的有效性得到了证实。

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