Papiha S S, Sertedaki A
Department of Human Genetics, University of Newcastle upon Tyne, UK.
Electrophoresis. 1995 Sep;16(9):1624-6. doi: 10.1002/elps.11501601268.
Following the development of DNA fingerprinting (Jeffreys et al., Nature 1985, 314, 67-73) using the minisatellite probes 33.15 and 33.6, many other fingerprinting probes have been described. The wide type M13 phage, 3' HVR region of alpha globin gene, F10 and various synthetic oligonucleotide probes have been shown to produce multiallelic and individual specific DNA fingerprints. The two probes developed by Jeffreys et al. have been extensively used in forensic and paternity determination in various laboratories, however, for the use of DNA fingerprints produced by synthetic oligonucleotide probes, still need critical evaluation. In this paper the statistical evaluations of the fingerprinting probes (CAC)5 was carried out by comparing the results produced by Jeffreys et al., using probes 33.15 and 33.6. The mutation rate calculated for (CAC)5 as 0.015 is nearly similar to the probe 33.15 (0.011) but higher than the probe 33.6 (0.005). The data obtained using (CAC)5 fingerprint in paternity cases from the northeast of England are presented.
继使用小卫星探针33.15和33.6开发出DNA指纹识别技术（杰弗里斯等人，《自然》1985年，第314卷，第67 - 73页）之后，又有许多其他指纹识别探针被描述出来。宽型M13噬菌体、α珠蛋白基因的3'高变区、F10以及各种合成寡核苷酸探针已被证明能产生多等位基因且具有个体特异性的DNA指纹。杰弗里斯等人开发的两种探针已在各个实验室广泛用于法医鉴定和亲子鉴定，然而，对于合成寡核苷酸探针产生的DNA指纹的应用，仍需要严格评估。本文通过比较杰弗里斯等人使用探针33.15和33.6得出的结果，对指纹识别探针（CAC）5进行了统计评估。计算得出（CAC）5的突变率为0.015，与探针33.15（0.011）相近，但高于探针33.6（0.005）。文中展示了在英格兰东北部亲子鉴定案例中使用（CAC）5指纹获得的数据。
