Scheid C, Koul H, Hill W A, Luber-Narod J, Jonassen J, Honeyman T, Kennington L, Kohli R, Hodapp J, Ayvazian P, Menon M
Department of Physiology, University of Massachusetts Medical School, Worcester 01655-0127, USA.
J Urol. 1996 Mar;155(3):1112-6.
The present studies assessed the possibility that high concentrations of oxalate may be toxic to renal epithelial cells.
Subconfluent cultures of LLC-PK1 cells were exposed to oxalate, and the effects on cell morphology, membrane permeability to vital dyes, DNA integrity and cell density were assessed.
Oxalate exposure produced time- and concentration-dependent changes in the light microscopic appearance of LLC-PK1 cells with higher concentrations ( > 140 microM.) inducing marked cytosolic vacuolization and nuclear pyknosis. Exposure to oxalate also increased membrane permeability to vital dyes, promoted DNA fragmentation and, at high concentrations (350 microM. free oxalate), induced a net loss of LLC-PK1 cells.
Since high concentrations of oxalate can be toxic to renal epithelial cells, hyperoxaluria may contribute to several forms of renal disease including both calcium stone disease and end-stage renal disease.
本研究评估了高浓度草酸盐可能对肾上皮细胞有毒性的可能性。
将亚汇合的LLC-PK1细胞培养物暴露于草酸盐中，并评估其对细胞形态、细胞膜对活性染料的通透性、DNA完整性和细胞密度的影响。
草酸盐暴露使LLC-PK1细胞的光学显微镜外观出现时间和浓度依赖性变化，较高浓度（>140微摩尔）可诱导明显的胞质空泡化和核固缩。暴露于草酸盐还增加了细胞膜对活性染料的通透性，促进了DNA片段化，并且在高浓度（350微摩尔游离草酸盐）下，导致LLC-PK1细胞净损失。
由于高浓度草酸盐可对肾上皮细胞有毒性，高草酸尿症可能导致多种形式的肾脏疾病，包括钙结石病和终末期肾病。
