一项通过新检测方法对由人细小病毒B19引起的传染性红斑暴发与非免疫性胎儿水肿之间关系的研究。

A study by means of a new assay of the relationship between an outbreak of erythema infectiosum and non-immune hydrops fetalis caused by human parvovirus B19.

作者信息

Yaegashi N, Okamura K, Tsunoda A, Nakamura M, Sugamura K, Yajima A

机构信息

Department of Obstetrics and Gynecology, Tohoku University School of Medicine, Sendai, Japan.

出版信息

J Infect. 1995 Nov;31(3):195-200. doi: 10.1016/s0163-4453(95)80026-3.

DOI:10.1016/s0163-4453(95)80026-3

PMID:8586838

Abstract

An enzyme-linked immunosorbent assay (ELISA), used to detect IgG and IgM antibody specific for human parvovirus B19, was established by use of human parvovirus B19 capsid protein VP-1 expressed in Escherichia coli. Paired samples of serum derived from 44 mothers and single samples derived from 24 babies having unexplained non-immune hydrops fetalis (NIHF), were tested by means of the assay. Five cases (11%) of NIHF were suspected of having been induced by intrauterine human parvovirus B19 infection because the samples of maternal serum were positive for parvovirus B19 IgM antibody. Four of the five cases arose during an outbreak of erythema infectiosum. According to our study and previous reports over 90% of NIHF caused by parvovirus B19 intrauterine infection have become clinically overt in the second trimester of pregnancy during a period ranging from 15 to 27 weeks of gestation.

摘要

利用在大肠杆菌中表达的人细小病毒B19衣壳蛋白VP - 1，建立了一种用于检测人细小病毒B19特异性IgG和IgM抗体的酶联免疫吸附测定（ELISA）法。采用该测定法对44名母亲的配对血清样本和24名患有不明原因非免疫性胎儿水肿（NIHF）的婴儿的单一样本进行了检测。5例（11%）NIHF疑似由宫内人细小病毒B19感染所致，因为母亲血清样本中细小病毒B19 IgM抗体呈阳性。5例中有4例发生在传染性红斑暴发期间。根据我们的研究及既往报告，超过90%由细小病毒B19宫内感染导致的NIHF在妊娠中期（妊娠15至27周）已出现临床症状。