HeLa细胞中的核糖体加工。前体核糖体和成熟核糖体结构方面的研究。
Ribosome processing in HeLa cells. Studies on structural aspects of precursor and mature ribosomes.
作者信息
Johnson T R, Kumar A
出版信息
J Cell Biol. 1977 May;73(2):419-27. doi: 10.1083/jcb.73.2.419.
Abstract
Mature ribosomes, their subunits, and their ribonucleoprotein precursors were extracted from HeLa cells and prepared for electron microscopy by a method based on adsorption of nucleic acid to a charged grid. Images so obtained revealed the presence of two types of ribonuclease-sensitive fibrils of consistent morphology. One of these types of fibril is present on the 80S and 55S nucleolar ribonucleoprotein precursors and the mature 60S subunit. The other type is restricted to the 80S precursor. The morphology of the fibrils and their distribution in the various preparations suggest identification with known secondary structural features of isolated pre-rRNA.
摘要
从海拉细胞中提取成熟核糖体、其亚基及其核糖核蛋白前体，并通过基于核酸吸附到带电荷网格的方法制备用于电子显微镜观察。如此获得的图像显示存在两种形态一致的核糖核酸酶敏感纤维。其中一种纤维存在于80S和55S核仁核糖核蛋白前体以及成熟的60S亚基上。另一种类型则仅限于80S前体。纤维的形态及其在各种制剂中的分布表明与分离的前体核糖体RNA的已知二级结构特征相符。
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