Liu H, Boas D A, Zhang Y, Yodh A G, Chance B
Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia 19104, USA.
Phys Med Biol. 1995 Nov;40(11):1983-93. doi: 10.1088/0031-9155/40/11/015.
In this article, we introduce a simple method to characterize optical properties and blood oxygenation in tissue using spatially resolved, steady-state reflectance. The method considers multiple source-detector separations larger than 2 cm, i.e. 20 times the optical mean free path in tissue, and makes an approximation to linearize the relationship between the separation and reflectance. Simulation results show that errors of the algorithm due to the approximation are less than 10%. Using a calibration sample, we calculate from the slope and intercept the absorption and reduced scattering coefficients, mu a and mu's, of a tissue-like solution, and experimental results confirm the usefulness of the method for quantitation of haemoglobin saturation in tissue.
在本文中，我们介绍了一种使用空间分辨稳态反射率来表征组织光学特性和血氧饱和度的简单方法。该方法考虑了多个大于2厘米的源探测器间距，即组织中光学平均自由程的20倍，并进行近似处理以使间距与反射率之间的关系线性化。模拟结果表明，由于近似处理导致的算法误差小于10%。使用校准样本，我们根据斜率和截距计算出类组织溶液的吸收系数和约化散射系数μa和μ's，实验结果证实了该方法在定量组织中血红蛋白饱和度方面的有效性。
