肌球蛋白轻链激酶的完整性对于肝星状细胞收缩至关重要。

Integrity of myosin light chain kinase is essential for Ito cell contraction.

作者信息

Watanabe S, Wang X E, Hirose M, Oide H, Shimizu H, Kitamura T, Yumoto A, Suzuki S, Miyazaki A, Sato N

机构信息

Juntendo University School of Medicine, Department of Gastroenterology, Tokyo, Japan.

出版信息

J Gastroenterol Hepatol. 1995;10 Suppl 1:S54-6. doi: 10.1111/j.1440-1746.1995.tb01799.x.

DOI:10.1111/j.1440-1746.1995.tb01799.x

PMID:8589344

Abstract

Hepatic sinusoidal Ito cells (fat storing cells) are believed to play a regulatory role on hepatic sinusoidal blood flow through their contraction. The detailed mechanism of contraction of Ito cells, however, is still unknown. The present study was undertaken to clarify the effect of new myosin light chain kinase inhibitor, wortmannin, on Ito cell contraction. Ito cells prepared from rat liver were cultured for 4 days before the study. The contraction of Ito cells, which was monitored and analysed by time-lapse video tape recording, was triggered by addition of endothelin-1. Wortmannin pretreatment for 1 h inhibited endothelin-induced Ito cell contraction dose-dependently. Therefore, the integrity of the actomyosin system is essential for Ito cell contraction and normal sinusoidal blood flow.

摘要

肝血窦伊托细胞（贮脂细胞）被认为通过其收缩对肝血窦血流起调节作用。然而，伊托细胞收缩的详细机制仍不清楚。本研究旨在阐明新型肌球蛋白轻链激酶抑制剂渥曼青霉素对伊托细胞收缩的影响。在研究前，将从大鼠肝脏分离的伊托细胞培养4天。通过延时录像监测和分析伊托细胞的收缩，其由添加内皮素-1触发。渥曼青霉素预处理1小时可剂量依赖性地抑制内皮素诱导的伊托细胞收缩。因此，肌动球蛋白系统的完整性对于伊托细胞收缩和正常的血窦血流至关重要。