Determination of 1,2,6-inositol trisphosphate (derivatives) in plasma using iron(III)-loaded adsorbents and capillary zone electrophoresis-(indirect) UV detection.
作者信息
Buscher B A, Tjaden U R, Irth H, Andersson E M, van der Greef J
机构信息
Division of Analytical Chemistry, Leiden/Amsterdam Center for Drug Research, University of Leiden, Netherlands.
出版信息
J Chromatogr A. 1995 Dec 22;718(2):413-9. doi: 10.1016/0021-9673(95)00701-6.
A method for the determination of 1,2,6-inositol trisphosphate (IP3) and derivatives in plasma by capillary zone electrophoresis with (indirect) UV detection has been developed. The sample pretreatment is based on the selective isolation after complexation of inositol phosphates with iron(III) loaded on an adsorbent. Plasma protein denaturation was performed with sodium dodecyl sulfate. The selectivity of the method is demonstrated with the analysis of phenylacetate-IP3. The recoveries amount to 65% and 88% in plasma and in water, respectively.
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