定位克隆简介。

Introduction to positional cloning.

作者信息

Papadopoulos N

机构信息

Johns Hopkins Oncology Center, Baltimore, Maryland, USA.

出版信息

Clin Exp Allergy. 1995 Nov;25 Suppl 2:116-8. doi: 10.1111/j.1365-2222.1995.tb00438.x.

DOI:10.1111/j.1365-2222.1995.tb00438.x

PMID:8590329

Abstract

The most common procedure for isolating genes that predispose to a certain disease is positional cloning. This process is initiated by mapping the responsible gene to its location on a chromosome linked to the disease. Successive cloning of the region results in the isolation of candidate genes. Finally, identification of mutations by mutational analysis points to the correct gene.

摘要

分离导致某种疾病的易感基因最常用的方法是定位克隆。这个过程首先要将相关基因定位到与该疾病相关的染色体上。对该区域进行连续克隆会分离出候选基因。最后，通过突变分析鉴定突变，从而确定正确的基因。