高效液相色谱-电化学检测法测定人血清和犬血浆中的巴尼地平。
Determination of barnidipine in human serum and dog plasma by HPLC with electrochemical detection.
作者信息
Takamura K, Abdel-Wadood H M, Kusu F, Rafaat I H, Saleh G A, el-Rabbat N A, Otagiri M
机构信息
School of Pharmacy, Tokyo University of Pharmacy and Life Science, Japan.
出版信息
Biol Pharm Bull. 1995 Oct;18(10):1311-4. doi: 10.1248/bpb.18.1311.
Barnidipine is a 1,4-dihydropyridine calcium antagonist. HPLC was conducted on a polybutadiene coated alumina column using an alkaline mobile phase and an electrochemical detector to determine the content of this drug in serum and plasma. A good linear relationship between barnidipine concentration and peak height was found in 5-500 ng/ml with a correlation coefficient of 0.998. The detection limit was 1 ng/ml. The within-day and day-to-day variations were examined for control human serum. Relative standard deviation of within-day assay for serum spiked with 10 ng/ml barnidipine.HCl was 6.9% and the recovery was 104%. A pharmacokinetic study was made in which the time course of barnidipine in dog plasma was followed.
巴尼地平是一种1,4 - 二氢吡啶类钙拮抗剂。采用碱性流动相和电化学检测器，在聚丁二烯涂覆的氧化铝柱上进行高效液相色谱法，以测定血清和血浆中该药物的含量。在5 - 500 ng/ml范围内，巴尼地平浓度与峰高之间呈现良好的线性关系，相关系数为0.998。检测限为1 ng/ml。对对照人血清进行了日内和日间变异检测。添加10 ng/ml盐酸巴尼地平的血清日内测定的相对标准偏差为6.9%，回收率为104%。进行了一项药代动力学研究，跟踪了巴尼地平在犬血浆中的时间进程。
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