Szymanski M, Barciszewska M, Erdmann V A, Barciszewski J
Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznan, Poland.
Biochem Mol Biol Int. 1995 Oct;37(3):413-22.
Comparative analysis of the nucleotide sequences of plant 5S rRNA genes shows numerous discrepancies with the mature 5S rRNAs. Detailed inspection of these differences revealed that they are mainly due to single nucleotide substitutions, insertions and deletions. In several cases also longer insertions were found, two of which are particularly interesting. Their location in loops resembles tRNA introns. One of these insertions is 79 nucleotides long and can potentially form a tRNA-like structure, with 50% homology to plant tRNAGly. This observation led us to propose that, in the maturation of some 5S rDNA transcripts, editing and splicing mechanisms may be involved.
对植物5S rRNA基因核苷酸序列的比较分析表明，其与成熟的5S rRNAs存在许多差异。对这些差异的详细检查发现，它们主要是由于单核苷酸替换、插入和缺失造成的。在一些情况下还发现了更长的插入片段，其中两个特别有趣。它们在环中的位置类似于tRNA内含子。其中一个插入片段长79个核苷酸，可能形成类似tRNA的结构，与植物tRNAGly有50%的同源性。这一观察结果使我们提出，在某些5S rDNA转录本的成熟过程中，可能涉及编辑和剪接机制。
