衰老与两栖类卵母细胞中的细胞信号改变有关吗？

Is aging related to cell signalling alterations in amphibian oocytes?

作者信息

Fonovich de Schroeder T M, de D'Angelo A M

机构信息

Libiquima, Facultad de Ingeniería, Universidad Nacional del Comahue, Buenos Aires, Neuquén, Argentina.

出版信息

Rev Bras Biol. 1995 Nov;55(4 Pt 2):865-9.

PMID:8599035

Abstract

The storage of Bufo arenarum oocytes decreased their ability to become fertilized "in vitro". The stimulation with carbachol of "young oocytes" showed a persistent hydrolysis with phosphatidylinositol 4,5 diphosphate (PIP2) while in "aged oocytes" both phosphatidylinositol 4 phosphate (PIP) and PIP2 were hydrolyzed at a non-significant level. These results and the lower 32P-phosphoinositide levels found in "aged oocytes" at time of stimulation agree with diminished phosphoinositide kinase and phospholipase C activities, as the consequence of a non-specific phosphoinositide hydrolysis probably occurring during storage.

摘要

中华蟾蜍卵母细胞的储存降低了它们“体外”受精的能力。用卡巴胆碱刺激“年轻卵母细胞”时，磷脂酰肌醇4,5 -二磷酸（PIP2）出现持续水解，而在“老化卵母细胞”中，磷脂酰肌醇4 -磷酸（PIP）和PIP2均以不显著的水平被水解。这些结果以及在刺激时“老化卵母细胞”中发现的较低的32P -磷酸肌醇水平，与磷酸肌醇激酶和磷脂酶C活性降低一致，这可能是储存期间可能发生的非特异性磷酸肌醇水解的结果。