白细胞介素2信使核糖核酸的一种结合因子。
A binding factor for interleukin 2 mRNA.
作者信息
Hua J, Paetkau V
机构信息
Department of Biochemistry, Medical Sciences Building, University of Alberta, Edmonton, Canada.
出版信息
Nucleic Acids Res. 1996 Mar 1;24(5):970-6. doi: 10.1093/nar/24.5.970.
Jurkat cells, a human T lymphocyte line that can be induced to synthesize and secrete interleukin 2, contain a factor that binds interleukin 2 mRNA. Binding can be demonstrated by formation of a complex detectable by gel electrophoresis. The binding is sequence specific and occurs in the 3'-non-coding region, within 160 nt of the end of the coding region, at or near a site on the mRNA that is rich in A and U residues. However, it appears not to be due to known AU binding factors. The factor is protease sensitive and binds non-covalently to interleukin 2 mRNA. It behaves like a protein of molecular weight 50 000-60 000 after UV-induced cross-linking to the mRNA. Preparations of the binding factor also protect interleukin 2 mRNA against degradation by a recently described RNasin-resistant endoribonuclease activity in Jurkat cells. Protection occurs under the same conditions required to generate the gel-retarded complex.
Jurkat细胞是一种可被诱导合成和分泌白细胞介素2的人T淋巴细胞系，它含有一种能与白细胞介素2信使核糖核酸（mRNA）结合的因子。这种结合可通过凝胶电泳检测到的复合物的形成来证明。该结合具有序列特异性，发生在3'-非编码区，位于编码区末端160个核苷酸内，在mRNA上富含A和U残基的位点或其附近。然而，这似乎并非由已知的AU结合因子所致。该因子对蛋白酶敏感，且与白细胞介素2 mRNA非共价结合。紫外线诱导其与mRNA交联后，它表现得像一种分子量为50000 - 60000的蛋白质。该结合因子的制剂还能保护白细胞介素2 mRNA免受Jurkat细胞中最近描述的一种对RNasin有抗性的核糖核酸内切酶活性的降解。在产生凝胶阻滞复合物所需的相同条件下即可发生保护作用。
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