Sang Q, Young H M
Department of Anatomy and Cell Biology, University of Melbourne, Parkville, 3052, Victoria, Australia.
Cell Tissue Res. 1996 Apr;284(1):39-53. doi: 10.1007/s004410050565.
Immunohistochemical techniques were used to examine the presence and co-localisation of a range of putative neurotransmitters and other neuronal markers in the myenteric plexus of the small and large intestine of the mouse. Distinct sub-populations of myenteric neurons were identified, based on the combinations of substances they contained and the distribution of their fibres. In the small intestine, there were two major classes of circular muscle motor neurons; one class was characterised by the presence of nitric oxide synthase, vasoactive intestinal peptide plus neuropeptide Y (NOS/VIP/NPY), and the second class contained calretinin plus substance P (CalR/SP). There were seven classes of neurons that innervated myenteric ganglia; these contained nos, vip, nos/vip, npy, calr/calbindin (calb), sp or 5-ht. In the large intestine, there were five major classes of motor neurons that contained nos, nos/vip, gaba, sp, or calr/sp, and seven major classes of neurons that innervated myenteric ganglia and contained nos, vip, calr/calb, calr, sp, gaba or 5-ht. Although some aspects of the patterns of co-localisation are similar to those in other species, this study re-inforces recent analyses that indicate significant species differences in neurochemical patterns in the enteric neurons of different species.
采用免疫组织化学技术检测小鼠小肠和大肠肌间神经丛中一系列假定神经递质及其他神经元标志物的存在及共定位情况。根据肌间神经元所含物质的组合及其纤维分布，确定了不同的亚群。在小肠中，有两类主要的环行肌运动神经元；一类以存在一氧化氮合酶、血管活性肠肽加神经肽Y（NOS/VIP/NPY）为特征，另一类含有钙视网膜蛋白加P物质（CalR/SP）。有七类神经元支配肌间神经节；这些神经元含有一氧化氮合酶、血管活性肠肽、一氧化氮合酶/血管活性肠肽、神经肽Y、钙视网膜蛋白/钙结合蛋白（calb）、P物质或5-羟色胺（5-ht）。在大肠中，有五类主要的运动神经元，分别含有一氧化氮合酶、一氧化氮合酶/血管活性肠肽、γ-氨基丁酸（GABA）、P物质或钙视网膜蛋白/P物质，以及七类主要的神经元支配肌间神经节，分别含有一氧化氮合酶、血管活性肠肽、钙视网膜蛋白/钙结合蛋白、钙视网膜蛋白、P物质、γ-氨基丁酸或5-羟色胺。尽管共定位模式的某些方面与其他物种相似，但本研究强化了最近的分析结果，即不同物种的肠神经元神经化学模式存在显著差异。
