Heiss S, Flicker S, Hamilton D A, Kraft D, Mascarenhas J P, Valenta R
Institute of General and Experimental Pathology, AKH, University of Vienna, Austria.
FEBS Lett. 1996 Mar 4;381(3):217-21. doi: 10.1016/0014-5793(96)00108-1.
Plant proteins belong to the most frequent elicitors of type I allergic symptoms in industrialized countries. Several relevant plant allergens have been found to be either specifically expressed or highly upregulated in mature pollen. The cDNA coding for a pollen specific maize protein, Zm13, shows significant sequence homology with a number of pollen or anther specific proteins from monocot and dicot plants as well as with recently described allergens from olive and rye grass. To test whether the Zm13 protein might possess IgE-binding capacity, Zm13 was expressed in E. coli. The coding region of Zm13 was PCR amplified from a genomic clone and expressed as a glutathione-S-transferase fusion protein. The recombinant Zm13 fusion protein bound a Zm13 specific rabbit antiserum and reacted with serum IgE from grass pollen allergic patients indicating that Zm13 and homologous proteins represent a family of conserved plant allergens.
在工业化国家，植物蛋白是引发I型过敏症状最常见的诱因之一。已发现几种相关的植物过敏原在成熟花粉中要么特异性表达，要么高度上调。编码花粉特异性玉米蛋白Zm13的cDNA与许多来自单子叶和双子叶植物的花粉或花药特异性蛋白以及最近描述的来自橄榄和黑麦草的过敏原具有显著的序列同源性。为了测试Zm13蛋白是否具有IgE结合能力，将Zm13在大肠杆菌中表达。从基因组克隆中通过PCR扩增Zm13的编码区，并将其表达为谷胱甘肽-S-转移酶融合蛋白。重组Zm13融合蛋白与Zm13特异性兔抗血清结合，并与草花粉过敏患者的血清IgE发生反应，这表明Zm13和同源蛋白代表了一类保守的植物过敏原。
